Mitocohdrial structure and function analysis of Ogura-type of CMS obtained in the progeny derived from interspecific chimera

通过本实验，可以获得以下结果，从而获得新的CMS诱导调控机制。发现了三个基因组分子，并明确了其结构。2.出版了内容。其诱导机制不是来自mt基因组重组，而是异质基因组的存在和化学计量比移位时的移位。嵌合体合成和原生质体融合过程均能诱导细胞质雄性不育的诱导和化学计量比漂移。这些诱导性Ogura类型的CMS包括碱基变化和微小的结构变化。事实表明，新的不育系诱导是可能的。本研究主要介绍了mt基因组及其相关分子的变化。此外，叶绿体基因组中出现了同源的基因组变化，特别是异质分子(基因组)的存在以及与CMS的漂移，表明整个控制细胞质基因组与核基因组有关。必须对系统基因组变化进行新的研究。白菜和甘蓝未发现恢复能力。这打乱了机理的研究和应用。利用细胞融合或属间杂交的方法进行Rf基因导入是控制Ogura-CMS的迫切需要。

Results described below could be obtained, such that new control mechanism of CMS induction may obtain by the present experiment..1. Three genome molecules were found and made clear the structures. The contents were published.2. The induction mechanism is not coming from mt genome recombination but heteroplasmic genome existence and the shift among those at stoichiometric shift..3. The CMS induction and stoichiometric shifts were induced both by chimera synthesis and protoplast fusion processes.4. These inductive Ogura-type of CMS included base changes and minor structural changes. The facts suggested the new CMS induction possibility.5. In the present study, mt genome and the related molecule changes were mainly shown. In addition, the homologous genome changes, especially heteroplasmic molecule (genome) existence and the shift with CMS occurred in chloroplast genome, suggesting whole control cytoplasmic genomes with nuclear genome. New research on the systematic genome change must be studied.6. Restoration ability has not been found in B.rapa and B.oleracea. That disrupted the mechanism research and application. One urgent necessity exists in the Rf gene introduction for the control Ogura-CMS by using cell fusion or intergeneric cross.