Analysis of shift in brain with film penetration peptide uniting enzyme for nerve type Gaucher disease treatment

证实用糖脑苷酶外观载体精制真核细胞来源的结合蛋白，并具有酶活性，大量合成蛋白，并对细胞内外运动进行检测。用生理盐水从小鼠尾骨静脉短时间给药，采用水动力法主要由肝脏进行强迫显现，并观察酶向血脑屏障的转移。此法因其简便、快捷、效果显著而受到近年来的重视。此外，本方法对质粒、酶、外观、内部、分布、确认、肽、包膜、渗透、活化、元素、单位、蛋白、脑、移、可能进行了研究。它成功地利用流体动力学方法使各种修饰葡萄糖脑苷酶-这种酶与肝脏一起出现，能够在静脉中快速施用。当身体内部运动被评估时，大脑的变化被检测到，尽管是很小的量。认为其含量与其他透膜肽的含量是一致的，有必要进行进一步的研究。

It is confirmed to refine the uniting protein of the eukaryocyte origin by using the appearance vector for glucocerebrosidase, and to have the enzymic activity, and a large amount of protein is made and the movement in the inside and outside of the cell is examined. The appearance plasmid is administered from mouse's coccygeal vein for a short time with the physiologic saline, the Hydrodynamic method to do the compulsion appearance by the liver chiefly is used, and the shift of the enzyme to the blood brain barrier is examined. This method is paid to attention recently because highly effective appearance is seen in easy and a short time. In addition, this.method.plasmid.enzyme.appearance.invivo.distribution.confirm.peptide.including.various.film.penetration.revitalization.element.unite.protein.brain.shift.possible. It succeeded in strong making various modification glucocerebrosidase-tat enzymes appear with the liver by using the Hydrodynamics method that was able to be administered rapidly in the vein. When the inside of the body movement had been evaluated, the shift in the brain was detected though was a small amount. It was thought that the amount and other film penetration peptide were united and a further examination was necessary in the future.