Basic study on suppression-effects of dental laser to bacterial biofilms

研究了Er：YAG激光对粪肠球菌、变形链球菌、粘性放线菌等4种单菌种的作用。采用改良的Robbins装置研究了痤疮丙酸杆菌在羟基磷灰石（HA）表面形成生物膜的情况。与对照组相比，在检查的所有组（20、40和80 mJ辐射）中，变形链球菌和粘性放线菌生物膜中的活细胞数量显著减少。20 mJ组痤疮丙酸杆菌和粪肠球菌细胞数无显著性差异。不含痤疮丙酸杆菌的3种细菌的生物膜形态发生改变，生物膜表面细胞较平。在灌注3种抗菌药物后0、1、3和7天，通过三磷酸腺苷（ATP）生物发光法进行抗菌作用，并通过扫描电子显微镜进行形态学研究。与对照组相比， ...更多信息 氯己定治疗组和对照组。细胞外基质结构和牙龈卟啉单胞菌细胞在氯己定的存在下改变。盐酸米诺环素还可引起牙龈卟啉单胞菌生物膜ATP含量降低和形态改变。甲硝唑对牙龈卟啉单胞菌生物膜无明显作用。研究了根管分离株（粪肠球菌、血链球菌、中间链球菌、化脓链球菌、金黄色葡萄球菌、具核梭杆菌、痤疮卟啉单胞菌、牙龈卟啉单胞菌和中间普氏菌）在牙胶尖上的初始生物膜形成能力。在补充有45或90 v%血清的培养基中孵育的标本表面上生成单一菌种粪肠球菌、血链球菌、中间链球菌、化脓性链球菌和金黄色葡萄球菌生物膜。粪肠球菌和血链球菌的生物膜显著厚于中间链球菌、化脓链球菌和金黄色链球菌。在与具核梭菌、痤疮丙酸杆菌、牙龈丙酸杆菌和中间型丙酸杆菌孵育的样本上未检测到生物膜。少

The effects of Er:YAG laser on 4 single species (Enterococcus faecalis, Streptococcus mutans, Actinomyces viscosus. Propionibacterium acnes) biofilms formed on hydroxyapatite (HA) disks using modified Robbins Device was studied. The numbers of viable cells in S.mutans and A.viscosus biofilm were significantly reduced in all groups examined (20, 40, and 80 mJ radiation) compare to control groups. However, The number of P.acnes and E.faecalis cells were shown no significant differences in 20 mJ group. The biofilms of 3 bacterial species without P.acnes were morphologically changed, and the surface cells of the biofilms were flat.Porphyromonas gingivalis strain 381 biofilms were prepared on HA disks. At 0, 1, 3, and 7 days after perfusion of 3 antimicrobial agents, the antimicrobial effects by adenosine triphosphate (ATP) bioluminescence, and morphological investigation by scanning electron microscopy was performed. A significant decrease (p < 0.001) in ATP content was found between the c … More hlorhexidine-treated and control groups. The extracellular matrix structure and P.gingivalis cells were altered in the presence of chlorhexidine. Minocycline hydrochloride also caused a decrease in the ATP content and morphological change on P.gingivalis biofilms. Metronidazole showed no significant efficacy against P.gingivalis biofilms.The initial biofilm-forming ability of root canal isolates (E.faecalis, Streptococcus sanguis, Streptococcus intermedius, Streptococcus pyogenes, Staphylococcus aureus, Fusobacterium nucleatum, P.acnes, P.gingivalis and Prevotella intermedia) on gutta-percha points was investigated. Single species E.faecalis, S.sanguis, S.intermedius, S.pyogenes and S.aureus biofilms were generated on the surfaces of the specimens incubated in culture mediums supplemented with 45 or 90 v% serum. The E.faecalis and S.sanguis biofilms were significantly thicker than those of S.intermedius, S.pyogenes and S.aureus. No biofilms were detected on the specimens incubated with F.nucleatum, P.acnes, P.gingivalis, and P.intermedia. Less