Development of with biological molecules immobilized titanium using tresyl chloride technique

我们的目标是将细胞黏附蛋白固定在钛上，以改善骨骼和组织的反应。钛表面的三氯甲苯处理能有效地将细胞黏附蛋白结合到钛表面。三苯基氯活化了钛表面的碱性羟基。钛表面氧化条件的不同并不影响细胞黏附蛋白在钛表面的固定化。通过X射线光电子能谱和傅里叶变换红外光谱的测量，证实了经三氯甲苯处理后钛表面碱性羟基的活化和细胞黏附蛋白在钛表面的固定。利用石英晶体微天平技术对钛表面结合蛋白的测定结果表明，采用三氯甲苯活化技术可以将更多的蛋白质固定在钛表面。半经验分子轨道计算表明，甲苯基氯的活化影响了前线分子轨道的能级。利用三氯甲苯活化技术将细胞黏附蛋白固定在钛表面，可提高人牙龈成纤维细胞在钛表面的初始黏附能力。因此，三氯甲苯活化技术是一种非常有用的钛固定化技术。

We aimed to immobilize the cell-adhesive proteins onto titanium for improving the bone and tissue response. Tresyl chloride treatment for titanium surface was effective for binding the cell-adhesive proteins onto titanium. Tresyl chloride activated the basic OH of titanium surface. The difference of oxidation conditions of titanium surfaces did not provide any influences for the immobilization of cell-adhesive protein onto titanium. The activation of basic OH of titanium surface by tresyl chloride treatment and the immobilization of cell-adhesive proteins onto titanium were confirmed by the measurement of X-ray photoelectron spectroscopy or Fourier-transform infrared spectroscopy. The measurement of binding proteins onto titanium using quartz-crystal microbalance technique revealed that greater amount of proteins could be immobilized onto titanium surface using tresyl chloride-activation technique. Semi-empirical molecular orbital calculation suggested that the tresyl chloride activation influenced the energy levels of frontier molecular orbital. The initial cell attachment of human gingival flbroblast to titanium was enhanced by the immobilization of cell-adhesive proteins onto titanium using tresyl chloride-activation technique.In conclusion, tresyl chloride-activation technique was very useful technique for the immobilization of cell-adhesive proteins onto titanium.