Development of with biological molecules immobilized titanium using tresyl chloride technique

我们的目的是将细胞粘附蛋白质固定在钛上，以改善骨和组织反应。对甲苯基氯处理钛表面是有效的结合细胞粘附蛋白到钛。对甲苯基氯活化了钛表面的碱性OH。钛表面氧化条件的不同对细胞粘附蛋白在钛上的固定没有任何影响。通过X射线光电子能谱和傅里叶变换红外光谱的测量，证实了三甲苯基氯处理钛表面的碱性OH的活化和细胞粘附蛋白质在钛上的固定。利用石英晶体微天平技术测定了蛋白质在钛表面的结合量，结果表明，利用三苯甲酰氯活化技术可以将更多的蛋白质固定在钛表面。半经验分子轨道计算表明，对甲苯基氯的活化作用影响前线分子轨道的能级。用三苯甲酰氯活化法将细胞粘附蛋白固定在钛上，可以促进人牙龈成纤维细胞在钛上的初始粘附，表明三苯甲酰氯活化法是一种非常有效的固定细胞粘附蛋白的方法。

We aimed to immobilize the cell-adhesive proteins onto titanium for improving the bone and tissue response. Tresyl chloride treatment for titanium surface was effective for binding the cell-adhesive proteins onto titanium. Tresyl chloride activated the basic OH of titanium surface. The difference of oxidation conditions of titanium surfaces did not provide any influences for the immobilization of cell-adhesive protein onto titanium. The activation of basic OH of titanium surface by tresyl chloride treatment and the immobilization of cell-adhesive proteins onto titanium were confirmed by the measurement of X-ray photoelectron spectroscopy or Fourier-transform infrared spectroscopy. The measurement of binding proteins onto titanium using quartz-crystal microbalance technique revealed that greater amount of proteins could be immobilized onto titanium surface using tresyl chloride-activation technique. Semi-empirical molecular orbital calculation suggested that the tresyl chloride activation influenced the energy levels of frontier molecular orbital. The initial cell attachment of human gingival flbroblast to titanium was enhanced by the immobilization of cell-adhesive proteins onto titanium using tresyl chloride-activation technique.In conclusion, tresyl chloride-activation technique was very useful technique for the immobilization of cell-adhesive proteins onto titanium.