Mechanisms of differentiation and maintenance of hematopoietic cells and their supporting microenvironment

1)浆细胞从脾向骨髓迁移的机制：为了弄清浆细胞向骨髓迁移的机制，我们对调控分子进行了研究。注射抗Flt3(A2F10)的拮抗剂可使小鼠骨髓浆细胞数减少，脾细胞数增加。抗原预刺激减少了对照组小鼠浆细胞上CXCL12的表达，但对A2F10处理组小鼠没有影响。通过Flt3在浆细胞上的信号传递减少了CXCL12，导致浆细胞加速向骨髓迁移。这些结果表明，F1t3信号对CXCL12表达的调控控制了骨髓浆细胞的数量。(手稿准备中)2)Wnt/β-连环蛋白信号通路在多能造血细胞中的作用。研究与干细胞自我更新有关的Wnt/β-连环蛋白信号通路的一个组成部分。逆转录病毒介导稳定的β-连环蛋白导入原始小鼠骨髓细胞…在基质细胞和细胞因子存在的情况下，更多的LS允许多潜能c-Kit^&lt；low&gt；Sca-1^&lt；low/-&gt；CD19^-CD11b^-Flt3^-CD43^+AA4.1^+NK1.1^-CD3^-CD11c^-Gr-1^-CD45R^+细胞的扩增。这些发现暗示了典型的Wnt通路信号在多能祖细胞的调节中。(J免疫学，2006,177：2294-2303)3)牙齿发育中多能细胞的特性为了评估牙间充质细胞在发育牙齿中的潜能，我们从E13.5牙胚细胞中制备间充质细胞，并在培养条件下评估其分化潜能。分化为成牙本质细胞、软骨细胞样细胞和成骨细胞样细胞。通过用P0-CRE/Rosa26R小鼠追踪NC来源的细胞作为LacZ^+细胞，证实了它们的来源。这些结果表明，在发育中的牙齿中存在有分化为软骨细胞样和成骨细胞样细胞的NC来源的细胞，这些细胞可能参与了牙齿的器官发生。(干细胞，2007，25：78-87)少

1)Mechanisms of the plasma cell migration from spleen to bone marrow.To be clear the mechanisms of migration of plasma cells into bone marrow, the regulatory molecule was assessed. An antagonistic antibody against Flt3 (A2F10) injection at priming reduced the numbers of plasma cells in bone marrow, and increased in spleen. Antigen priming reduced the expression of CXCL12 on plasma cells in control mice, but not in A2F10-treated mice. Signaling via Flt3 on plasma cells reduced CXCL12, resulting in accelerating the migration of plasma cells to bone marrow. These results suggested that the regulation of CXCL12 expression by F1t3 signaling controlled plasma cell numbers in bone marrow. (Manuscript in preparation)2)Function of Wnt/β-catenin signaling for multipotent hematopoietic cells.To investigate one component of the Wnt/β-catenin signaling pathway that has been implicated in stem cell self-renewal. Retroviral-mediated introduction of stable β-catenin to primitive murine bone marrow cel … More ls allowed the expansion of multipotential c-Kit^<low>Sca-1^<low/->CD19^-CD11b^-Flt3^-CD43^+AA4.1^+NK1.1^-CD3^- CD11c^-Gr-1^-CD45R^+ cells in the presence of stromal cells and cytokines. These findings implicated the canonical Wnt pathway signaling in regulation of multipotent progenitors. (J. Immunol, 2006, 177: 2294-2303)3)Characterization of multipotent cells in developing teeth.To assess the potential of dental mesenchymal cells in developing teeth, we prepared mesenchymal cells from E13.5 tooth germ cells and assessed their potential for differentiation in culture. They differentiated into odontoblasts, chondrocyte-like cells, and osteoblast-like cells. Their derivation was confirmed by tracing NC-derived cells as LacZ^+ cells using P0-Cre/Rosa26R mice. These results suggest that NC-derived cells with the potential to differentiate into chondrocyte-like and osteoblast-like cells are present in the developing tooth, and these cells may contribute to tooth organogenesis. (Stem Cells, 2007, 25 : 78-87) Less