The effect on promoter activities of the PPAR by CLOCK/BMAL1

脂质吸收和代谢受摄食和昼夜节律系统的调节。有研究认为，参与脂质代谢的酶的表达受生物钟系统的直接控制。本研究旨在检测过氧化物酶体增殖激活受体（PPAR）的启动子活性是否通过CLOCK/BMAL1异源二聚体具有转录活性。8-12周龄的雄性小鼠在实验开始前至少两周保持在12.12小时的光暗循环下。在肠内检测BMAL1和PPAR的mRNA谱。体外荧光素酶法检测CLOCK/BMAL1对PPAR启动子活性的直接影响。PPAR靶基因的表达随BMAL1的表达而周期性变化。CLOCK/BMAL1的表达增加了PPAR启动子的活性。删除PPAR启动子后，CLOCK/BMAL1不影响转录激活。PPAR transactivates。

Lipid absorption and metabolism are regulated by feeding and by the circadian system. It has been suggested that the expression of enzymes involved in lipid metabolism is directly controlled by the clock system. This study was designed to examine whether or not the promoter activities of peroxisome proliferator-activated receptor (PPAR) has transcriptional activity via the CLOCK/BMAL1 heterodimer. Male mice 8-12 weeks old were maintained under a 12 : 12 hour light-dark cycle for at least two weeks before the day of the experiment. The mRNA profiles of BMAL1 and of the PPAR were measured in intestine. The direct effects of CLOCK/BMAL1 on the promoter activities of PPAR were assessed in vitro by luciferase assay. The expression of PPAR target genes changed in a cyclical manner that followed expression of BMAL1. The promoter activities of the PPAR were increased by CLOCK/BMAL1 expression. After deletion of the promoter of PPAR, CLOCK/BMAL1 did not affect transactivation. PPAR transactivates.