Investigation for the etiologic agents of Kawasaki syndrome using Protein Chip analysis

为了探讨川崎综合征（KS）的病因，我们对KS患者血清蛋白质谱进行了研究。患者与方法：8例KS患者治疗前血清和8例年龄匹配的发热患者血清。为了简化分析条件，这些血清是从疾病早期和年龄小于6个月的KS患者中收集的。结果：在KS和对照组之间观察到显著差异的250个峰中，选择20个峰作为潜在的生物标志物。认为两种蛋白质代表在不同条件下在KS患者血清中观察到的11个增加的峰;在阳离子交换芯片（pH 4）中具有质荷比（m/z）的峰：11，524和11，581。KS的这些峰值强度与患病日期、KS症状数量、白色血细胞计数、C反应蛋白值或转氨酶值无关。在KS中9个降低的峰中，铜片（pH 7）中m/z 28，008的峰显示最宽的ROC面积。然后，为了证实这一事实，我们用另一个样品（8名KS患者和5名对照血清）研究了血清蛋白谱。结果：在pH 4的阳离子交换芯片上，KS与对照组在m/z 11，524和11，631峰上无差异。然而，使用16个KS样品和13个对照样品，在阳离子交换芯片（pH 4）中具有m/z 17389、17，405、14，052、8，702和14，056的峰在组之间显著不同。在这些峰值中，有峰值升高，在急性期的KS.Conclusions，虽然没有检测到KS的病原体，候选人KS的生物标志物已被选中。需要进一步检查。

In order to find the etiologic agents of Kawasaki syndrome (KS), we investigated the serum protein profiles of patients with KS. Patients and Methods: Sera of 8 KS patients before treatment and 8 age matched febrile patients were used. To simplify the analysis conditions, these sera were collected from the KS patients at an early phase of the disease and younger than 6 months of age. Results: Among 250 peaks with an observed significant difference between KS and control patients, 20 peaks were selected as potential biomarkers. Two proteins were considered to represent 11 increased peaks observed in different conditions in the sera of KS patients; peak with mass-to-charge ratios (m/z): 11,524 and 11,581 in the cation exchange chip (pH4). These peak intensities in KS showed no correlation with the day of illness, number of KS symptoms, white blood cell counts, values of C-reactive protein, or values of transaminases. Among 9 decreased peak in KS, a peak with m/z 28,008 in the copper chip (pH7) showed the widest ROC area. These peaks were considered potential biomarkers of KS.Then, to confirm this fact, we investigated the serum protein profiles using another samples (sera of 8 KS patients and 5 controls). Results: There were no differences between KS and control groups in the peaks of m/z 11,524 or 11,631 in the cation exchange chip (pH4). However, using 16 KS samples and 13 control samples, peaks with m/z 17389, 17,405, 14,052, 8,702, and 14,056 in the cation exchange chip (pH4) were significantly different between the groups. In these peaks, there were peaks that elevated in the acute phase of KS.Conclusions; Although etiologic agents of KS were not detected, candidates for biomarkers of KS have been selected. Further examination is necessary.