Systematic analysis of physiological and anti-oxidative function of L-ascorbic acid

1)从实验和计算化学的角度对l -抗坏血酸（=AsA，维生素C）及其相关化合物（如脱氢-AsA，单脱氢-AsA， 2,3-二酮- l -谷氨酸酸等）进行了详细的结构分析，并提供了分析这些化合物在实际反应现场真实动态结构的基础重要数据。2)采用实验化学和计算化学等多种方法研究了AsA及其相关化合物与三态氧分子的反应活性，获得了主要反应产物和反应机理的基本数据。3)作为一种模型酶反应体系，研究了丙基羟化酶对胶原分子中l -脯氨酸残基的羟基化反应，并概述了AsA在胶原分子生物合成过程中的作用。4)为了阐明被动吸烟这一典型氧化应激对生物系统的影响，进行了多项动物实验。本实验以不能合成AsA的ods大鼠为实验动物，深入研究了被动吸烟和AsA给药对这些动物主要器官（肝脏和肾脏）抗氧化酶基因表达的影响，获得了一些重要的基础数据，为系统分析AsA的生理和抗氧化功能提供了必要的基础数据。5)为完成本报告，我们对上述动物实验结果进行了再次确认，并利用DPPH自由基试剂进行了进一步的实验，首次阐明了AsA清除自由基功能的新反应机理，这是AsA抗氧化机制的基础。

1) The detailed structural analyses of L-Ascorbic acid (=AsA, Vitamin C) and its related compounds (ex. Dehydro-AsA, monodehydro-AsA, 2,3-diketo-L-gulonic acid, etc.) were carried out from the view point of both experimental and computational chemistry, and basic important data available to analyze the real dynamic structure of these compounds in the actual reaction site.2) The reactivity of AsA and its related compounds with the triplet state oxygen molecule was studied by using the various methods of both experimental and computational chemistry, and the basic data about the major reaction products and reaction mechanisms were obtained.3) As a model enzyme reaction system, hydroxylation reaction of L-proline residue in collagen molecule by prorylhydroxylase was studied, and the outlines of the role of AsA in the biosynthesis process of collagen molecule were elucidated.4) To clarify the effect of passive smoking, a typical oxidative stress, on the biological systems, several animal experiments were carried out. In these experiments, ODS-rats which were unable to synthesize AsA were used as the experimental animals, and the effects of passive smoking and AsA administration on the gene expression of anti-oxidative enzymes in the major organs (Livers and kidneys) of these animals were intensively investigated and some important basic data which were necessary for the systematic analysis of both physiological and anti-oxidative function of AsA were obtained.5) To make this report, re-confirmation of the results of animal experiments described above were made and further additional experiments using DPPH radical reagent were also made, and a new reaction mechanism of radical scavenging function of AsA, the basis of anti-oxidation mechanism of AsA, were clarified for the first time.