Biosynthesis of bacterial cellulose utilizing biomass waste as a recycled resource towards a generation of a sustainable society.

我们已经从环境中分离出了耐盐的细菌纤维素生产微生物。该研究项目的目的是将含盐生物质废弃物作为纤维素纤维的再生资源进行再利用，并通过添加纤维再生高质量的纸张。菌株523是一株耐盐纤维素产生菌，能在含1.5%NaCl的培养基中存活，并在培养基表面产生纤维素纤维。尽管向再生纸中添加细菌纤维素对于改善废纸的质量是有效的，但是在由非耐盐乙酸细菌制备的纤维素纤维的情况下，菌株523的纤维素合成低于乙酸细菌。因此，在2004财政年度，对培养基成分、通气和其他理化条件进行了检查，以提高纤维素产量。基于这些结果，在液体培养中获得了能够高纤维素合成的改良培养物。根据系统发育分析和生理特性，将菌株523确定为中华根瘤菌属（Sinorhizobium sp.）的一个种，与醋酸菌不同，菌株523能在NaCl存在下合成较细的纤维素纤维。我们的研究结果揭示了纤维素纤维作为一种可能的材料的未来应用，以及通过将生物质废物作为再生资源来产生可持续发展的社会。

We have isolated halotolerant, bacterial cellulose-producing microbes from environments. The purpose of the research project is to reuse salt-containing biomass waste as a recycled resource of cellulose fiber, and also to regenerate high quality paper by adding the fiber. Strain 523 is a halotolerant cellulose producing bacteria that can survive in a medium containing 1.5% NaCl and produced cellulose fiber on the surface of the medium. Although the addition of the bacterial cellulose to the recycled paper is effective for improvement of the quality of the waste paper, as in the case of the cellulose fiber made by the non-halotolerant acetic acid bacteria, cellulose synthesis of strain 523 was lower than acetic acid bacteria. Therefore, in the 2004 fiscal year, culture condition for higher production of the cellulose was examined on the constituent of media, aeration, and other physiochemical conditions. Based on these results, the improved culture which enables high cellulose synthesis was obtained in a liquid culture. Based on the phylogenetic analysis and physiological characteristics, strain 523 was designated as a species in the genus Sinorhizobium.Unlike acetic acid bacteria, Sinorhizobium sp.strain 523 enables to synthesis thinner cellulose fibers in the presence of NaCl. Our results revealed the future application of the cellulose fiber for a possible material and that the generation of a sustainable society by using biomass waste as a recycled resource.