Molecular phylogenic study of Ehrlichia spp. microorganisms as the etiological agents of emerging diseases in Japan

1)在日本北海道某莱姆病流行区捕获的小鼠类中检测到埃利希体基因。利用针对埃利希体属的168个rDNA引物对和沙非伊蚊和鼠伊蚊的16S rDNA的其他区域进行鉴定。在94只阿姬鼠中，有4只检测到特异的16S rDNA片段。73只鼠中有5只（阳性率为6.8%）。扩增的168 rDNA片段的核苷酸序列与产自日本东北部的E - muris-like ehrlichia、E spp HF565和Shizuoka-36最为相似。基于16S rDNA序列的系统发育分析表明，东北和西南两个类群与E. muris类群形成一个集群。结果表明，日本存在两类muris样埃利希菌。2)在苏丹遗传检测到一种新的心水病原体——鲁曼埃利希菌基因型。新基因型暂时命名为“Gedaref”。对该菌株pCS20和主抗原蛋白I （map）基因的系统发育分析结果表明，该菌株属于E. ruminantium-群，但与已知的E. ruminantium菌株不对应。此外，在图谱上的系统发育分析结果显示，“Gedaref”与苏丹唯一的反刍大肠杆菌菌株Um Banein外的其他菌株形成集群。这项工作为苏丹不仅存在乌姆巴内菌株，而且存在其他菌株提供了证据。

1)Ehrlichial gene was detected from small rodents trapped in a Lyme disease-endemic area in Hokkaido, the northernmost island of Japan. Primer pairs of 168 rDNA targeting the genus of Ehrlichia and other region of the 16S rDNA specific for E. chaffeensis and E. muris were used for identification. The DNA fragment specific for 16S rDNA of E. spp. was detected from 4 of 94 Apodemus. speciosus mice (positive rate 4.3%) and 5 of 73 Clethrionomys rufocanus bedfordiae mice (positive rate 6.8%). The nucleotide sequence of the amplified 168 rDNA fragment was most similar to those of E muris-like ehrlichia, E spp HF565 and Shizuoka-36 originating in the northeastern part of Japan. In phylogenic analysis based on 16S rDNA sequences, these northeastern and southwestern groups of E. murrslike ehrlichia made a cluster with E. muris group microorganisms. These results suggest that there are two groups of E. murislike microorganisms in Japan.2)A novel genotype of Ehrlichia rummantium, the causative agent of heartwater, was genetically detected in the Sudan. The new genotype was provisionally named as "Gedaref". The results of phylogenic analyses on the regions of pCS20 and major antigen protein I (map)) genes revealed that the ehrlichia) strain belongs to E. ruminantium-group, but does not correspond with any known strains of E. ruminantium. Furthermore, as the results of phylogenic analyses on map), "Gedaref" made clusters with several strains of E. ruminantium other than Um Banein, which had been known as the sole strain of E. ruminantium in the Sudan. This work provided the evidence that not only Um Banein strain but also the other strain exist in the Sudan.