MORPHOLOGICAL ANALYSIS OF THE LYMPHATIC DRAINAGE OF THE ASCiTIC FLUID AND THE METASTATIC MECHANISM OF PERITONEAL

通过壁层腹膜淋巴管系统的吸收途径对于了解腹腔和盆腔淋巴转移具有重要的临床意义。此外，在prietal腹膜淋巴网络的形态特征尚未澄清。以前，我们证明了酶组织化学的结构组织的血管和毛细血管在几种哺乳动物组织。本研究应用5 '-核苷酸酶（5'-Nase）-碱性磷酸酶（ALTH）组织化学方法和腹腔注射超细碳粒（CH-40，21 nm），在整体标本或OCT冰冻切片中观察壁层腹膜纤维的结构和分布。本研究使用了15只日本猴（Macaca fuscata）。采用5 '-Nase-ALP双重染色的整体包埋法，可清楚区分毛细淋巴管和毛细血管。发育良好的5 '-Na ...更多信息 在膈到腹膜区的过渡区和肾周脂肪区局部观察到Se阳性淋巴网。这些管-囊状神经管的特征是不规则的网状网络。前区和前外侧区的腹膜分支及其网络数目较前区迅速减少。在直肠膀胱和直肠子宫（道格拉斯囊）袋中，观察到由管状囊泡组成的树状网络。在腹膜前区的原始神经末梢顶端出现大量的结节状末梢。在道格拉斯囊内，卵巢悬韧带周围有发达的淋巴管网。本研究首次用酶组织化学方法证实了5 ′-Nase阳性淋巴管在猴腹膜壁层的精确分布。这些发现表明，腹膜淋巴网络作为另外的途径可能在临床上在从腹膜腔吸收腹水和颗粒物质中起重要作用。少

Absorptive pathway via the lymphatic vessels system in the parietal peritoneum is clinically important for understanding lymphatic metastasis in the abdominal and pelvic cavities. Moreover, morphological characteristics of lymphatic networks in the prietal peritoneum have not yet been clarified. Previously we demonstrated enzyme-histochemically the structural organization of the lymphatics and blood capillary in several mammalian tissues. The present study is to describe the structure and distribution of the parietal peritoneal lymphatics in the whole-mount preparations or OCT frozen sections using histochemical method 5'-nucleotidase (5'-Nase) -alkaline phosphatase (ALPase) and intraperitoneal injection with ultra-fine carbon particles (CH-40, 21nm). Fifteen Japanese monkeys (Macaca fuscata) were used in this study. The whole-mount preparation with 5'-Nase -ALPase double staining enabled us to differentiate clearly lymphatic capillaries from the blood capillaries. Well-developed 5'-Na … More se-positive lymphatic networks were locally observed in the transitional area from the diaphragm to the peiitoneal regions and in the perirenal fat region. These tubo-saccular lymphatics were characterized by an irregular mesh-like network. The number of peritoneal lymphatics and their networks in the anterior and anterolateral regions rapidly decreased as compared with that in the foregoing regions. In the rectovesical and rectouterina (Douglas pouch) pouches, a tree-like network consisting of the tubulo-saccular lymphatics was observed. Numerous knob-like endings appeared at the apical parts of the initial lymphatics in the anterior peritoneal region. In Douglas pouch, a fine dense lymphatic network was well-developed around the suspensory ligament of ovary. The present study has first demonstrated enzyme-histochemically the precise distribution of 5'-Nase-positive lymphatic vessels in the monkey parietal peritoneum. These findings suggest a possibility that the peiitoneal lymphatic networks, as an additional pathway may clinically play an important role in absorption of ascitic fluid and particulate matter from the peritoneal cavity. Less