Pathogenesis of Kawasaki disease : Gene expression in susceptible hosts determined by microarray

背景：尽管广泛的研究，川崎病（KD）的病因仍然不明。我们的假设表明，非常常见的感染性病原体可能会导致某些易感宿主的KD。我们利用Affyssin基因芯片系统研究了KD患者和对照组基因表达的差异。研究方法：5例川崎病患者，年龄~岁，平均随访时间年，其中A组8例有冠状动脉病变，B组B例无冠状动脉病变，另2例无川崎病病史者作为对照。我们用外周白色血细胞中单个核细胞提供的RNA样品分析了基因表达谱。基因表达差异达1.5倍以上，差异显著。结果：KD患儿基因表达谱多数相似。但3组间基因表达存在一定差异。人胚系IgD链基因和人T细胞的mRNA ...更多信息 细胞白血病/淋巴瘤1在伴CAL的KD患者中的表达高于不伴CAL的KD患者。KIAA 0752蛋白基因在伴GALS的KD患者中的表达高于不伴CALS的KD患者。KD伴GALS组HLA-DR 7和HLA-ILA-DRw 53的连接基因表达高于无KD史组。人补体细胞溶解抑制因子（CLI）mRNA和精脒/精胺N1-乙酰转移酶（SS-A）基因在无KD病史的KD患者中的表达明显高于伴CAL的KD患者。SS-A基因可能与血管疾病有关。以往的研究表明，人脐静脉内皮细胞中SS-A基因的表达与切应力有关。结论：遗传因素可能参与了KD的发病，尤其与血管病变有关。目前的结果表明，进一步调查的变化作为基线信息的来源，用于设计未来的遗传治疗的冠状动脉病变由于KD血管炎的优点。少

Background : The etiology of Kawasaki disease (KD) remains unknown despite extensive investigations. Our hypothesis suggested that very common infectious agents might cause KD in some susceptible hosts. We investigated the difference of gene expression between patients with KD and control subjects by use the Affymetrix GeneChip system. Methods : Five patients with KD, ranged age from to years, mean follow up periods years, including Group A patients (n=8) who had coronary artery lesions GALs and Group B patients (n=8) who had no GALs, 2 patients who had no history of KD as controls (n=10), were studied. We analyzed a gene expression profile with RNA sample provided mononuclear cells in peripheral white blood cells. The difference of gene expression did more than 1.5 times with significance. Result : Most gene expression in KD patients was resembled. However some differences of gene expression were found among 3 groups. The gene of Human gremline IgD chain gene and H.sapiens mRNA for T … More cell lerkemia/lymphoma 1 were expressed on KD patients with CALs more than on patients with no CALs. In addition, the gene of Homo sapiens mRNA for KIAA0752 protein were expressed on KD patients with GALS more than on KD patients with no CALS. The connection gene of a Human MHC class II HLA-DR7 and Human MHC class II I-ILA-DRw53 were expressed on KD patients with GALS more than patients who had no history of KD. The gene of Human complement cytolysis inhibitor (CLI) mRNA and Spermidine/Spermine N1-Acetyltransferase (SS-A) were expression on patients without history of KD more than KD patients with CALs. SS-A gene may relate to vascular disease. Previous report suggested that gene expression of the SS-A related to the shear stress in human umbilical vein endothelial cells. Conclusion : This study suggested that some hereditary factors may participate in KD patients, especially to relate the factors of vascular disease. The present results demonstrate the merit of further investigation of the alterations as a source of baseline information for the design of future genetic therapy for coronary artery lesions due to KD vasculitis. Less