Development of enclosed body of Free-Radical material by way of the efficacious reinforcement at the hyper-thermia therapy for peritonitis carcinomatosa

我们已经证实，使用含有多不饱和脂肪酸的5-FU栓剂化疗，对直肠癌局部复发具有与温热治疗和放射治疗相同的杀瘤作用。在本项目中，我们计划开发含有多不饱和脂肪酸的微球，这种脂肪酸在高温下产生自由基物质，并应用于晚期直肠癌腹膜炎的超热化疗。二十碳五烯酸是多不饱和脂肪酸。它被称为欧米茄-3脂肪酸(或n-3多不饱和脂肪酸)。它有预防血栓形成的作用，它能降低甘油三酯，它有抗炎作用，还可以抑制癌症。我们不能合成它。在20℃或37℃时为液态。我们将在体外用单克隆抗体进行定量测定，但我们测定的结果不稳定。我们认为它不能保持物质的平衡，也不能稳定单抗。我们将添加抗氧化剂或改变多不饱和脂肪酸的相对分子质量或侧链。如果我们能够测量多不饱和脂肪酸，我们将测量腹膜内注射时向腹膜淋巴组织的转移率，并假设对其他器官有副作用。

We already confirmed that chemotherapy using 5-FU suppository, contained poly-unsaturated fatty acid, had the tumorcidal effect with hyper-thermo therapy and radiation therapy at local recurrent of rectal cancer. In this project, we planed that we would develop microsheres contained poly-unsaturated fatty acid, this fatty acid made Free-Radical material affected by hyperthermia, and we would apply for the hyper-thermo-chemo-therapy at the peritonitis carcinomatosa with advance rectal cancer.The eicosapentaenoic acid is poly-unsaturated fatty acid. And it calls Omega-3 fatty acid (or n-3 poly-unsaturated fatty acid). It has preventation of a thrombosis, it is gone down triglyceride, it has anti-inflammation action, and it may suppress cancer. We cannot synthesize it. It is a liquid state at 20℃ or 37℃. We will measure it quantitatively using mono-clonal antibody in vitro, but we measured unstable. We think it cannot keep balance of substance, or stabilizing of mono-clonal antibody. We will add antioxidants or change molecular weight or side chain of poly-unsaturated fatty acid.If we are able to measure poly-unsaturated fatty acid, we will measure transfer rate to lymphoid tissue on the peritoneum at Intraperitoneal injection, and presuppose side effect on other organ.