Identification of novel autoantibodies against cerebral cell surface membrane proteins as biological markers of multiple sclerosis

鉴定新型抗脑细胞表面膜蛋白自身抗体作为多发性硬化症的生物标志物

• 批准号: 20590992
• 负责人: KIMURA Akio
• 金额: $ 2.91万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590992/
• 关键词: 脳・神経; 免疫学; autoantibody; Heat shock protein 70; multiple sclerosis (MS); proteome; Phoshoglyceate mutase 1(PGAMI); Stress-70 protein(mtHSP70); 多発性硬化症; 視神経脊髄炎; 自己抗体; Phosphoglycerate mutase 1; stress 70 protein; 通常型多発性硬化症; 75 kDa glucose-regu

We identified the autoantibody against Phosphoglycerate mutase 1 (PGAM 1) and mitochondrial heat shock protein 70 (mtHSP70) in serum from multiple sclerosis (MS) patients by immunoproteomic approach. We tested more samples by one-dimensional immunoblotting of human recombinant PGAM1 and mtHSP70. The prevalence of the anti-PGAM 1 antibody is significantly higher in serum from MS patients (81%) than in serum from other neurological diseases (ONDs) and healthy controls (HCs) (24～32%) (p<0.05). The prevalence of the anti-mtHSP70 antibody is also significantly higher in serum from MS patients (68%) than in serum from ONDs and HCs (20～30%) (p<0.05). We studied the clinical features and magnetic resonance imaging findings of MS patients with these antibodies. As a result, there were no significant differences between these antibodies-positive and -negative MS patients. Additionally, in our comprehensive analysis of the prevalence of both the anti-PGAM1 antibody and the anti-mtHSP70 antibody, the positivity rates of both these antibodies were significantly higher in serum from MS patients (57%) than in serum from patients with ONDs and HCs (0～16%) (p<0.05) ; moreover, the specificity of this combination assay was higher than that of the assay of only one antibody (57% sensitivity ; 93% specificity). Results of our study suggest that the combination of both these antibodies is useful for a more specific diagnosis of MS.

应用免疫蛋白质组学方法检测多发性硬化（MS）患者血清中抗磷酸甘油酸酯β 1（PGAM 1）和线粒体热休克蛋白70（mtHSP 70）的自身抗体。我们测试了更多的样品，通过一维免疫印迹的人重组PGAM 1和mtHSP 70。MS患者血清中抗PGAM 1抗体阳性率（81%）显著高于其他神经系统疾病（OND）和健康对照（HC）（24~32%）（p<0.05）。抗mtHSP 70抗体在MS患者血清中的阳性率（68%）也显著高于OND和HC患者（20~30%）（p<0.05）。我们研究了这些抗体的MS患者的临床特征和磁共振成像结果。因此，这些抗体阳性和阴性MS患者之间没有显着差异。综合分析抗-PGAM 1抗体和抗-mtHSP 70抗体在MS患者血清中的阳性率（57%）显著高于OND和HC患者（0~16%）。（p<0.05）;此外，该组合测定的特异性高于仅一种抗体的测定（57%灵敏度; 93%特异性）。我们的研究结果表明，这两种抗体的组合是有用的MS的更具体的诊断。

项目成果

Motor-dominant CIDP患者の臨床的検討

运动主导型 CIDP 患者的临床回顾

• 发表时间: 2010
• 作者: 木村暁夫;櫻井岳郎;山田恵;香村彰宏;林祐一;田中優司;保住功;犬塚貴
• 通讯作者: 犬塚貴

Antibodies to the GABAB receptor in limbic encephalitis with seizures : case series and characterization of the antigen.

边缘叶脑炎伴癫痫发作的 GABAB 受体抗体：病例系列和抗原特征。

• 发表时间: 2010
• 期刊: Lancet Neurol. 9
• 作者: Lancaster E;Lai M;Peng X;Hughes E;Constantinescu R;Raizer J;Friedman D;Skeen MB;Grisold W;Kimura A;Ohta K;Iizuka T;Guzman M;Graus F;Moss SJ;Balice-Gordon R;Dalmau J
• 通讯作者: Dalmau J

Serial monitoring of basal metabolic rate for therapeutic evaluation in an Issacs' syndrome patient with chronic fluctuating symptoms

连续监测基础代谢率以评估患有慢性波动症状的伊萨克斯综合征患者的治疗效果

• 发表时间: 2010
• 期刊: Internal medicine 49
• 作者: Hayashi Y;Kimura A;Watanabe N;Yamada M;Sakurai T;Tanaka Y;Hozumi I;Inuzuka T
• 通讯作者: Inuzuka T

Identification of antibodies as biological markers in serum from multiple sclerosis patients by immunoproteomic approach

• DOI: 10.1016/j.jneuroim.2010.11.003
• 发表时间: 2011-04-01
• 期刊: JOURNAL OF NEUROIMMUNOLOGY
• 影响因子: 3.3
• 作者: Sakurai, Takeo;Kimura, Akio;Inuzuka, Takashi
• 通讯作者: Inuzuka, Takashi

Selective cauda equine hypertrophy with idiopathic inflammation.

选择性马尾肥大伴特发性炎症。

• 发表时间: 2008
• 期刊: Muscle Nerve. 38
• 作者: Hayashi Y;Sakurai T;Kimura A;Ikeda T;Matsuyama Z;Suzuki Y;Tanaka Y;Hozumi I;Hosoe H;Takahashi H;Inuzuka T
• 通讯作者: Inuzuka T