Detection of candidate antigen of Acanthamoeba castellanii practicable for immunological examination of Amoebic Keratitis

可用于阿米巴角膜炎免疫学检查的卡氏棘阿米巴候选抗原的检测

• 批准号: 23590836
• 负责人: KIMURA Akio
• 金额: $ 3.33万
• 依托单位: Osaka Prefectural Institute of Public Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590836/
• 关键词: Acanthamoeba; 細胞障害性; アカントアメーバ; 特異抗原蛋白質

We investigated cytopathic effect of Acanthamoeba T2, T4 and T7 genotypes on human corneal epithelial cell (HCE-T). Cytopathic effects were observed not only in T4 genotype but also in T2 genotypes that classified as nonpathogenic species by 18SrRNA genotyping. These results suggested the discrepancy between 18sRNA typing and actual pathogenicity of Acanthamoeba species. In addition, we found that protease inhibitor did not inhibit cytopathic effect of Acanthameoba on HCE-T cell, and after challenging Acanthamoeba to rabbit kidney 13 cell induced interferon via interferon-sensitive response element. These results suggested the existence of novel mechanism of Acanthamoeba keratitis. To investigate antigen which might be useful for diagnosis of amoebic keratitis, we constructed cDNA library of A. castellanii and screened immunologically using polyclonal rabbit antibody against A. castellanii soluble antigen. However, in spite of our expectation, we could not obtain positive clones.

研究了阿米巴T2、T4和T7基因型对人角膜上皮细胞（HCE-T）的致细胞病变作用。细胞病变不仅发生在T4基因型中，而且发生在18 SrRNA基因分型为非致病种的T2基因型中。这些结果表明，18 sRNA分型与阿米巴的实际致病性之间存在差异。另外，我们发现蛋白酶抑制剂不能抑制阿米巴对HCE-T细胞的致细胞病变作用，并且阿米巴攻击兔肾13细胞后，通过干扰素敏感反应元件诱导干扰素。提示阿米巴角膜炎可能存在新的发病机制。为寻找阿米巴角膜炎的诊断抗原，构建了阿米巴角膜炎的cDNA文库。castellanii的多克隆抗体进行免疫学筛选。卡氏菌可溶性抗原然而，尽管我们的期望，我们不能获得阳性克隆。

项目成果

AcanthamoebaおよびNaegleriaの分子分類と今後の課題

棘阿米巴和耐格里阿米巴的分子分类及未来的挑战

• 发表时间: 2011
• 作者: 木村明生;枝川亜希子;倉田貴子;楠原康弘
• 通讯作者: 楠原康弘

レジオネラ属菌のアメーバ内増殖能を利用したアメーバ共培養法の検討

利用军团菌在阿米巴体内增殖的能力检查阿米巴共培养方法

• 发表时间: 2012
• 作者: 枝川亜希子;木村明生;田中榮次;足立伸一;宮本比呂志
• 通讯作者: 宮本比呂志

AcanthamoebaおよびNaegaleria属の分子分類の現状と今後の課題

棘阿米巴属和耐加拉虫属分子分类的现状和未来挑战

• 发表时间: 2011
• 作者: 木村明生;枝川亜希子;倉田貴子;楠原康弘
• 通讯作者: 楠原康弘