Identification of novel anti-neural and anti-endothelial cell antibodies for diagnosis and therapy of autoimmune-mediated encephalitis

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We identified novel anti-neural and anti-endothelial cell antibodies in serum and cerebrospinal fluid samples from patients with autoimmune-mediated encephalitis, who had no previous reported anti-neural and anti-endothelial cell antibodies. To identify these novel antibodies, we employed two-dimensional immunoblotting or immunoprecepitation using human neuroblastoma cells, human brain microvascular endothelial cells, or rat brain homogenate as the antigen source. As a result, we identified antibodies against intracellular adhesion molecule 5 in serum from a patient with Hashimoto encephalitis, ryanodine receptor 1 in serum from a patient with paraneoplastic cerebellar degeneration, and myosin regulatory light chain 12A in cerebrospinal fluid from a patient with paraneoplastic limbic encephalitis.