The research about overexpression gene identification with neuroblastoma on Biochip

本研究计划首先利用生物芯片技术对神经母细胞瘤过表达基因进行筛选。然而，从技术上讲，由于不可能，决定用基因芯片来鉴定参与自发分化和退化机制的基因。神经母细胞瘤是已知的儿童最常见的实体瘤之一，1岁以下儿童常表现为自发分化和退化。但有些转移到不好的类型，进展到有转移的晚期。因此，了解哪个肿瘤会消退或进展是非常重要的。采用成熟度组织学分类方法，对14例鼻咽癌组织的基因表达谱进行分析，以阐明其自发分化和消退的机制。计算机分析确定了该基因，该基因在分化较差的神经母细胞瘤和分化较差的神经母细胞瘤之间存在差异。分化的NB组包括与细胞成熟和凋亡相关的基因。与肿瘤发生有关的低分化NB组。未来，将增加检查；它认为我们希望能够导致新的治疗策略的发展。

This research planned to fine overexpression gene of neuroblastoma by using Biochip at first. However, technically, because it was impossible, it decided to identify the gene which participates in the mechanism of spontaneous differentiation and regression with cDNA microarray.Neuroblastoma (NB), one of the most common solid tumors among children, is known found under the age of one year often show spontaneous differentiation and regression. But some shift to unfavorable type and progress to an advance stage with metastasis. Therefore, it is very important to know which tumor will regress or progress. Using histological classified by the degree of maturation, we analyzed gene-expression profiles of 14 NB tumors on coda microarray to elucidate the mechanisms of spontaneous differentiation and regression. Computational analysis identified the gene which has a difference between differentiating NB tumors and poorly differentiated NB tumors. Differentiating NB group included genes associated with cell maturation and apoptosis. Poorly differentiated NB group included in relation to tumorigenesis. The future, examination will be added ; it thinks that we want to be able to lead to development of new therapeutic strategies.