Alteration of salivary IgA level due to surgical stress

结果表明，手术损伤对免疫系统产生各种变化，包括外周淋巴细胞数量减少，自然杀伤活性降低，免疫球蛋白的变化。本研究旨在探讨手术损伤对膜免疫的影响。观察大鼠唾液IgA分泌量的变化及唾液IgA产生量的变化。（材料与方法）(1)采用ICR雄性小鼠在全身麻醉下进行手术。结合皮肤切口和皮瓣的发展是一种手术侮辱。在给予匹罗卡平（毒蕈碱受体激动剂）的情况下收集唾液，用微移液管从口腔中收集唾液。未治疗的小鼠在戊巴比妥麻醉下采集唾液作为对照。(2) ELISA法测定唾液样品IgA浓度。(3)竞争PCR法测定IgA mRNA。（结果）术后唾液中IgA浓度立即升高，次日恢复正常。当以每唾液蛋白IgA浓度计算该值时，该值在第二天呈高趋势，但术后立即不高。这一结果表明，IgA在机体防御手术损伤方面起作用。术后即刻颌下腺IgA mRNA含量升高。（结论）提示手术损伤后交感神经占优势的情况下，IgA的产生和分泌增加。

It was shown that surgical insult exerts various changes on an immunity system, which include reduction of the number of peripheral lymphocytes, reduction of natural killer activity, change of an immunoglobulin. The purpose of the study is to examine the influence on the membrane immunity due to surgical insult. Quantitative change of secretion of salivary IgA, and change of the amount of IgA production in the salivary gland were examined.(Materials and Method) (1) Operation was carried out under general anesthesia using the ICR male mice. A combination of a skin incision and development of a flap was employed as a surgical insult. Collection of saliva was performed with administration of pilocarpine (muscarinic receptor agonist), and saliva was collected with micro pipette from the mouth. Untreated mice were used for saliva collection under pentobarbital anesthesia as a control. (2) IgA concentration of saliva sample was determined by ELISA. (3) IgA mRNA was determined by competitive PCR.(Results) The IgA concentration in the saliva rose immediately after surgery, ad returned to the normal value after the next day. When this value was calculated as IgA concentration per saliva protein, it showed tendency to be high on the next day, but not high immediately after surgery. This result suggests that IgA was working for body defense against the surgical insult.The amount of IgA mRNA in the submandibular gland was increased immediately after surgery.(Conclusion) It was suggested that increase of production and secretion of IgA was induced by the condition that the sympathetic nerve was predominant due t surgical insult.