Basic Research for Small Caliber Regenerative Graft Development Aiming Clinical Application

1.PAU（聚氨基甲酸酯）包被的亲水性小口径再生移植物的制作及犬模型的建立，以评价该新型移植物的有效性：包被3mm直径的移植物并用于犬颈动脉植入模型，但遗憾的是由于吻合口内膜增生，很少获得可用于评估的专利移植物。专利移植物用于评估。包被的移植物在宏观上呈组织性，在显微镜下其内层被较少的血栓和内皮样细胞覆盖。骨髓源性内皮祖细胞在体外包被PAU、细胞外基质和生长因子的聚四氟乙烯（PTFE）片上粘附和增殖的最佳条件确定：利用G-CSF动员外周血单个核细胞测定培养板中PAU/ECM在聚四氟乙烯（PTFE）片上的组合。6种ECM， I型和IV型胶原蛋白，纤维连接蛋白，2纤维连接蛋白肽和玻璃体连接蛋白用于PAU/ECM组合。在PAU/ECM组合中，“I型胶原+纤维连接蛋白- grgd肽+玻璃连接蛋白”对G-CSF动员的外周血单个核细胞在“聚四氟乙烯片”上的增殖效果最好，其次是“纤维连接蛋白+玻璃连接蛋白”。基于实验“2”数据的新型小口径再生接枝包覆PAU/ECM组合的评价：尚不清楚。

1.Production of PAU (Polyaminourethane)-coated hydrophilic small caliber regenerative graft and creation of canine model for judging the effectiveness of this new graft : 3mm diameter graft was coated and used for canine cervical artery implantation model, but unfortunately few patent grafts were obtained for evaluation due to intimal hyperplasia of anastomotic site. The patent grafts ware used for evaluation. The PAU-coated graft was histotropic macroscopically and inner-layer of it was covered with less thrombus and endothelial-like cell microscopically.2.Determination of optimal conditions of bone marrow derived endothelial progenitor cell adhesion and proliferation on "PTFE (Polytetrafluoroethylene) sheets" coated with PAU, ECM (extracellular matrix), and growth factors in vitro : G-CSF mobilized peripheral-blood mononuclear cells were used for determination of PAU/ECM combinations on "PTFE sheets" in culture plates. Six ECM, type I & IV Collagen, Fibronectin, 2 Fibronectin peptide and Vitronectin were used for making PAU/ECM combinations. Among PAU/ECM combinations, "type I Collagen+Fibronectin-GRGD peptide+Vitronectin" was most efficient, and "Fibronectin+Vitronectin" was next for G-CSF mobilized peripheral-blood mononuclear cell proliferation on "PTFE sheets".3.Evaluation of the new small caliber regenerative graft coated with PAU/ECM combination based on the data of experiment "2" : It remains to be unclear.