Bone Marrow Cell Culture for Producing Tissue Engineered Regenerative Blood Vessels as a Goal of Clinical Application
Bone Marrow Cell Culture for Producing Tissue Engineered Regenerative Blood Vessels as a Goal of Clinical Application
批准号:
14580812
负责人:
ISHIDA Atsushi
金额:
$2.24万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2002
资助国家:
日本
项目状态:
已结题
起止时间:
2002 至 2003
中文摘要
1.确定有效、简便的骨髓血管再生细胞群分选方法:针对临床应用,结合近期其他研究资料,认为G-CSF动员外周血单个核细胞可替代“骨髓血管再生细胞群”,用于实验“2”2。体外联合骨髓细胞、生长因子和人工细胞外基质(ECM)三种血管再生不可缺少的元素,确定短期“血管片”的最佳制备条件:本研究的主题。在以I型和IV型胶原蛋白、纤维连接蛋白、玻璃体连接蛋白和纤维蛋白原为ECM的培养基中,观察到骨髓来源的CD34阳性细胞在培养板上的内皮定植。治疗1年后,纤维连接蛋白的疗效是其他ECM的5倍。第二年,为了进一步进行体外血管三维培养再生,采用临床常用的血管假体之一聚四氟乙烯(PTFE)片作为细胞培养“支架”,用PAU(聚氨基甲酸乙酯)包被,使其具有亲水性,增加组织亲和力,骨髓来源的CD34阳性细胞和G-CSF动员的外周血单个核细胞。采用6种ECM、I型和IV型胶原蛋白、纤维连接蛋白、2型纤维连接蛋白肽和玻璃体连接蛋白,观察其在三维培养体系中对细胞粘附和增殖的加速作用,其中以纤维连接蛋白RGD肽最有效。分析“血管片”中内皮细胞与平滑肌细胞的转分化机制尚不清楚。
英文摘要
1.Determination of effective and simple cell-groups sorting for vessel regeneration from bone marrow cells : Aiming at clinical applications, based on recent other research data, G-CSF mobilized peripheral-blood mononuclear cells are thought to be substituted for "cell-groups for vessel regeneration from bone marrow cells" and used for experiment "2"2.Determination of optimal conditions for making "vascular sheets" shortly In vitrocombining three inevitable elements, bone marrow cells, growth factors and artificial extracellular matrix (ECM) for vessel regeneration : Main theme of this study. Endothelial colonization of bone marrow derived CD34 positive cells in culture plates was observed in the media with type I & IV Collagen, Fibronectin, Vitronectin and Fibrinogen as ECM. It was obtained at 1st year that efficacy of Fibronectin was 5 times of those of other ECM. At 2nd year, aiming at further ex vivo vessel regeneration with 3 dimensional culture, one of clinically used vascular prosthesis, PTFE (Polytetrafluoroethylene) sheets, were used for cell culture "scaffold" after coating with PAU (Polyaminourethane) to make hydrophilic and to increase tissue affinity, Bone marrow derived CD34 positive cells and G-CSF mobilized peripheral-blood mononuclear cells were used. Six ECM, type I & IV Collagen, Fibronectin, 2 Fibronectin peptide and Vitronectin were used for observation of acceleration of cell adhesion and proliferation in 3 dimensional culture system Among them, Fibronectin RGD peptide was most efficient.3.To analyze the mechanism of transdifferenciation between endothelial cells and smooth muscle cells in "vascular sheets" It remains to be unclear.
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石田厚: "ハイブリッド型人工血管の開発のため、骨髄由来内皮前駆細胞が生着・増殖に効果的な多孔質ポリ4フッ化エチレン樹脂(ePTFE)人工マトリックスの開発"千葉医学雑誌(0303-5476). 79巻・6号. 265 (2003)
Atsushi Ishida:“开发一种多孔聚四氟乙烯树脂(ePTFE)人工基质,可有效促进骨髓源性内皮祖细胞的植入和增殖,从而开发混合人工血管”《千叶医学杂志》(0303-5476)。第 79 卷第 6 期。265 (2003)
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通讯作者:
Ishida, A.: "Development of Polytetrafluoroethylene (PTFE) artificial Matrix for effective adhesion and proliferation of Bone Marrow derived Endothelial Progenitor Cells to produce Hybrid-type prosthetic vascular graft"Journal of the Chiba Medical Society
Ishida, A.:“开发聚四氟乙烯 (PTFE) 人工基质,使骨髓源性内皮祖细胞有效粘附和增殖,以生产混合型人工血管移植物”千叶医学会杂志
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石田厚, 今牧瑞浦, 宮崎勝: "ハイブリッド型人工血管の開発のため、骨髄由来内皮前駆細胞が生着・増殖に効果的なPTFE人工matrixの開発"日本血管外科学会雑誌(0918-6778). 12巻・3号. 312 (2003)
Atsushi Ishida、Mizuura Imamaki、Masaru Miyazaki:“开发一种 PTFE 人造基质,可有效促进骨髓源性内皮祖细胞的植入和增殖,从而开发混合人造血管”日本血管外科学会杂志( 0918-6778) )。第 12 卷,第 3 期。312 (2003)。
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石田厚: "ハイブリッド型人工血管の開発のため、骨髄由来内皮前駆細胞が生着・増殖に効果的な多孔質ポリ4フッ化エチレン樹脂(ePTFE)人工マトリックスの開発"千葉医科雑誌(0303-5476). 79巻・6号. 265 (2003)
Atsushi Ishida:“开发一种多孔聚四氟乙烯树脂(ePTFE)人工基质,可有效促进骨髓源性内皮祖细胞的植入和增殖,从而开发混合人工血管”《千叶医学杂志》(0303-5476)。第 79 卷第 6 期。265 (2003)
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Ishida, A., Imamaki, M., Hirano, M., Masuda, M., Miyazaki, M.: "Bone Marrow Cell Culture for therapeutic angiogenesis with cell implantation as a clinical application"Journal of Japanese Society for Vascular Surgery. 11-2. 321 (2002)
Ishida, A.、Imamaki, M.、Hirano, M.、Masuda, M.、Miyazaki, M.:“骨髓细胞培养与细胞植入治疗性血管生成的临床应用”日本血管外科学会杂志。
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