Clarification of cause wheat protein in exercise-induced anaphylaxis with respect to wheat processed food

将小麦蛋白分离为盐溶、乙醇和碱溶蛋白，以明确小麦依赖运动诱发过敏反应的主要过敏原。用大鼠制备针对每种蛋白的特异性抗血清。与其他分离蛋白相比，盐溶蛋白对胃蛋白酶的消化率更高。ICR小鼠在负荷跑台上跑30min后，分别给ICR小鼠灌胃各分离蛋白，测定其在消化管中的残留量。各分离蛋白均保持抗原性，在小肠的可溶性内容物中检测到抗原性。此外，在每个门静脉血中检测到每个保持抗原性的蛋白。B10.口服每种蛋白质后，用每种分离蛋白致敏的小鼠在跑步机上跑步。力竭跑后所有致敏小鼠的肠道侵蚀程度均高于未致敏小鼠。此外，在…后进行30min的适度运动口服更多的每种小麦分离蛋白也会导致醇溶蛋白和麦谷蛋白组的肠道侵蚀。此外，我们观察到醇溶蛋白和谷蛋白从肠道泄漏到肝脏。这些结果表明，小麦依赖运动过敏反应的主要因素可能是小麦蛋白中的醇溶蛋白和麦谷蛋白。虽然食物过敏原通常是可溶的，但Gliadin是小麦依赖运动过敏反应的主要过敏原，不溶于盐溶液。说明不溶性醇溶蛋白是在胆汁乳化条件下被酶消化后顺利吸收并保持抗原性的。未致敏小鼠口服各分离蛋白后，检测各蛋白的消化率和吸收情况。小鼠口服醇溶蛋白后可见明显的胃肠粘膜损伤，H-E染色可见小鼠小肠绒毛明显脱落。对于这一结果，提示醇溶蛋白消化产生的某些多肽与乳糜泻的致病多肽相同，对小肠粘膜有毒性作用。此外，演习造成了更严重的破坏。较少

The wheat protein was fractionated in the salt soluble, EtOH soluble and alkali soluble proteins to clarify the main allergen concerning the wheat dependent exercise-induced anaphylaxis. Specific antisera to each protein was prepared by using the rat. The digestibility of the salt soluble protein with the pepsin advanced comparing the other fractionated proteins. After ICR mouse was administered each fractionated protein and loaded treadmill running for 30 min, the protein remained in a digestive canal was measured. Each fractionated protein maintained the antigenicity was detected in the soluble content of small intestines. In addition, each protein maintained antigenicity was detected in each portal blood. B10.A mouse sensitized with each fractionated protein was run on a treadmill after oral intake each protein. The level of intestinal erosion was higher in all sensitized mice than that in the unsensitized ones after exhaustive running. Further more, moderate exercise for 30 min aft … More er oral ingestion of each wheat-fractionated protein also induced intestinal erosion in the gliadin- and glutenin-groups. In addition, we observed leaking of gliadin and glutenin proteins out of the intestine into the liver. These results indicated that the main factor involved in wheat-dependent exercise-induced anaphylaxis might be the gliadin and glutenin in wheat proteins.Gliadin, a major allergen of wheat-dependent exercise-induced anaphylaxis, is insoluble in a salt solution, though food allergens are generally soluble. It was suggested that insoluble gliadin was digested by protease under the emulsified condition of bile and smoothly absorbed holding the antigenic activity. After the orally administrate of each fractionated protein to unsensitized mouse, the digestibility and absorption of each protein were examined. The gastrointestinal mucosal lesion was remarkably observed in the mouse oral administrated gliadin and remarkable dropout of the villus of the mouse small intestine was admitted by the H-E dye. As for this result, it is suggested that some peptide generated by gliadin digestion identical with a cause peptide of the celiac disease to have the toxicity action on the small intestines mucous. Moreover, the exercise made the damage more seriously. Less