Research for wound healing using an artificial skin model composed with cultured human skin cells.

使用培养的人皮肤细胞制成的人工皮肤培养物作为实验模型以及临床应用已经引起了关注。然而，人造皮肤几乎没有发生损伤和观察到进展的化学制品。我们制作了许多人造皮肤模型。用不同强度的CO2激光照射这些人工模型。我们用病理学和免疫组化技术评价了这些模型的损伤程度和组织再生的时间过程。然后，在短时间内通过未受损的表皮角质形成细胞的生长来修复受损的表面。相反，当真皮层受损时，角质形成细胞再生需要较长的时间。从这些结果中，我们得出结论，照射皮肤部位的表皮角质形成细胞的存活是重要的皮肤组织再生。当激光照射是温和的并且角质形成细胞保留在照射区域时，表皮角质形成细胞发育多层并且修复的表皮厚而坚固。相反，当真皮细胞受损时，修复的表皮较弱，表皮角质形成细胞层较少。我们还通过使用激光照射的人工皮肤模型研究了人胎盘提取物的作用。用CO2激光照射样品，并在含有1%HPE、0.1%HPE、0%HPE（对照）的培养基中培养。结论是，HPE产生了特定的密度影响作为伤口愈合的加速剂，但连续量的HPE产生了特定的密度影响作为inhibitory.Our观察类似于生理伤口愈合过程中的活体。我们相信，这种人工皮肤模型是独特的，并为各种激光照射后的皮肤再生的实验研究有用。

Artificial skin cultures made using cultured human skin cells have attracted attention as experimental models, as well as for clinical applications. However artificial skin has few chemical articles that undergo damage and observed progress. We created a number of artificial skin models. These artificial models were irradiated with a CO2 laser at various strengths. We evaluated the degree of damage and the time course of tissue regeneration in these models using pathological and immunohistochemical techniques.With mild irradiation conditions, epidermal keratinocytes in the deep layer survived. The damaged surface was then repaired by the growth of undamaged epidermal keratinocytes in a short time. In contrast, when the dermal layer was damaged, a longer time period was required for keratinocyte regeneration. From these results, we conclude that surviving epidermal keratinocytes in irradiated skin sites are important for skin tissue regeneration. When laser irradiation is mild and keratinocytes remain at the irradiated area, epidermal keratinocytes develop many layers and the repaired epidermis is thick and firm. In contrast, when the dermal cells are damaged, the repaired epidermis is weaker with fewer layers of epidermal keratinocytes.We also studied the effects of human placental extract by using an artificial skin model with laser irradiation. The samples were irradiated with a CO2 laser and cultured in a medium with 1% HPE, 0.1% HPE, 0% HPE (control). The conclusion was that HPE produced a specific density affect as an accelerator of wound healing, but successive amounts of HPE produced a specific density affect as an inhibitor.Our observations resemble physiological wound healing processes within the living body. We believe that this artificial skin model is unique and useful for experimental studies of skin regeneration after irradiation with various lasers.