Production of volatile organic compounds (VOCs) by marine bacteria

本文对一株海洋细菌Alteromonasmacleodii产生CH_3I的潜力进行了研究。在海洋肉汤2216中，加入或不加入1 mmol/L碘化钾，分别在10、15、20、25、30 ℃下培养细菌。在15 ~ 30℃范围内，培养温度越高，增殖速度越快。碘化钾对细胞增殖率无影响。用碘化钾在15 ~ 30℃之间生成CH_3I。CH_3I产生的最适温度为25℃。A.在25℃条件下，用0.1 ~ 1000μmol/L碘化钾对macleodii进行培养。结果表明，碘离子浓度越高，CH_3I的生成量越大。当碘化钾浓度超过10μmol/L时，CH_3I的生成量显著增加。在表层海水碘浓度约为0.4μ mol/L时，产生的CH_3I约为0.2μmol/L。这表明，海洋细菌在表层海水中可产生约2- 20%的CH_3I（培养基中细菌总数是表层海水中的100-1000倍）。

We evaluated the potential of a marine bacterium, Alteromonas macleodii, to produce CH_3I. Bacterium was incubated at temperatures (10,15,20,25,30℃) in marine broth 2216 with or without 1 mmol/L of potassium iodide. From 15 to 30℃, the higher the culture temperature, the more rapid the proliferation rate. Proliferation rate was not affected by potassium iodide. CH_3I was produced between 15 and 30℃ with potassium iodide. Optimum temperature for CH_3I production was 25℃. A. macleodii was also incubated at 25℃ in medium spiked with potassium iodide from 0.1 to 1000μmol/L. Results showed that the greater the concentration of spiked iodide, the more CH_3I was produced. Notably, production of CH_3I strongly increased at over 10μmol/L of potassium iodide. At the concentration of iodine in surface seawater (about 0.4μmon/L), the CH_3I produced was estimated to be about 0.2μmol/L. This suggested that marine bacteria would produce about 2-20 % of CH_3I in surface seawater (the total number of bacteria in medium was 100-1000 times higher than that seen in surface seawater).