Examination of estrogenic potencies of each enantiomer on marine fish larva and influence analysis to resources

对来自日本的四个种群进行了组织学检查，以评估性腺病变的可能性。在羽田附近和东京湾的富津附近，在佐治岛附近的相模湾和滨奈子海湾都捕到了鱼。羽田附近的地点与之前报道的女性化比目鱼的地点一致。我们还分析了鱼的血清类固醇和鱼的肌肉中壬基酚的浓度。在羽田采集的73只雄性中，有3只发现了双性特征，如核周卵母细胞的存在，但在其他地点的雄性中没有发现。在羽田采集的2只雌性和从滨海角采集的2只雌性卵巢中发现了充血样病理。这两种病理与血清类固醇激素水平或鱼的大小都不相关。羽田海域采集到的雄鱼体内的壬基酚平均浓度显著高于相模湾采集的雄鱼的平均浓度。总而言之，这些发现表明，东京湾一些地区的鱼类正常配子发生可能受到内分泌干扰，而且这个问题可能比目前认识到的更普遍。开发了一种使用气相色谱制备组分收集器来分离手性化合物对映体的自动化分离系统。该系统消除了通过气相色谱分离后的人工收集程序的需要。为了评价该系统的有效性，我们将其应用于对映体的分离和收集。-六氯环己烷，我们测量了每个对映体的雌激素活性。两种对映体都是。-六氯环己烷表现出类似的雌激素活性。该分析方法同样适用于其他手性化合物的雌激素活性的测定。这种测定是必不可少的，因为对映体在生物体中积累和在环境中分解的方式有很大的不同。

A histological examination was conducted to assess the possibility of gonadal pathologies in four populations of konoshiro gizzard shad from Japan. Fish were collected off Haneda and off Futtsu in Tokyo Bay, off Zushi in Sagami Bay, and in Hamanako Sound. The location off Haneda corresponds to the site where feminized flounder have been previously reported. We also analyzed serum steroids in fish and muscle concentration of nonylphenol in fish. Intersex characteristics, such as the presence of perinucleolar oocytes, were found in 3 out of 73 males collected off Haneda but not in males from the other sites. Congestion-like pathologies were found in the ovaries of 2 females collected off Haneda and 2 from Sound Hamanako. Neither of these pathologies was correlated with the serum steroid hormone levels or with the size of the fish. The average concentration of nonylphenol in males collected off Haneda was significantly larger than that of males from Sagami Bay. Collectively, these findings suggest the possibility of endocrine disruption of normal gametogenesis in fish from some areas of Tokyo Bay and that this problem could be more widespread than currently recognized.An automated enantiomeric isolation system was developed using a gas chromatograph-preparative fraction collector to separate the enantiomers of chiral compounds. This system eliminate the need for a manual collection procedure after separation by gas chromatography. To evaluate the usefulness of this system we applied it to the separation and collection of the enantiomers of. -hexachlorocyclohexane, and we' measured the estrogenic potency of each enantiomer. Both enantiomers of. -HCH showed similar estrogenic activity. We concluded that this analytical method is suitable for the determination of the estrogenic activity of other chiral compounds. Such determinations are essential because enantiomers differ considerably in how they accumulate in organisms and decompose in the environment.