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Examination of estrogenic potencies of each enantiomer on marine fish larva and influence analysis to resources

各对映体对海水鱼类幼体的雌激素效力测定及对资源的影响分析

基本信息

批准号：
12660164
负责人：
HASHIMOTO Shinya
金额：
$ 2.62万
依托单位：
University of Shizuoka (2001)東京水産大学 (2000)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

A histological examination was conducted to assess the possibility of gonadal pathologies in four populations of konoshiro gizzard shad from Japan. Fish were collected off Haneda and off Futtsu in Tokyo Bay, off Zushi in Sagami Bay, and in Hamanako Sound. The location off Haneda corresponds to the site where feminized flounder have been previously reported. We also analyzed serum steroids in fish and muscle concentration of nonylphenol in fish. Intersex characteristics, such as the presence of perinucleolar oocytes, were found in 3 out of 73 males collected off Haneda but not in males from the other sites. Congestion-like pathologies were found in the ovaries of 2 females collected off Haneda and 2 from Sound Hamanako. Neither of these pathologies was correlated with the serum steroid hormone levels or with the size of the fish. The average concentration of nonylphenol in males collected off Haneda was significantly larger than that of males from Sagami Bay. Collectively, these findings suggest the possibility of endocrine disruption of normal gametogenesis in fish from some areas of Tokyo Bay and that this problem could be more widespread than currently recognized.An automated enantiomeric isolation system was developed using a gas chromatograph-preparative fraction collector to separate the enantiomers of chiral compounds. This system eliminate the need for a manual collection procedure after separation by gas chromatography. To evaluate the usefulness of this system we applied it to the separation and collection of the enantiomers of. -hexachlorocyclohexane, and we' measured the estrogenic potency of each enantiomer. Both enantiomers of. -HCH showed similar estrogenic activity. We concluded that this analytical method is suitable for the determination of the estrogenic activity of other chiral compounds. Such determinations are essential because enantiomers differ considerably in how they accumulate in organisms and decompose in the environment.

对来自日本的四个Konoshiro砂鱼种群进行组织学检查以评估性腺病理的可能性。在东京湾的羽田和Futtsu附近、相模湾的祖师附近和Hamanako Sound附近采集鱼类。羽田外海的位置与先前报道的雌性化比目鱼的地点相对应。我们还分析了鱼的血清类固醇和肌肉中壬基酚的浓度。间性特征，如核仁周围卵母细胞的存在下，被发现在3的73名男性收集了羽田，但没有在男性从其他网站。在Haneda和Sound Hamanako采集的2只雌性动物的卵巢中发现充血样病理。这些病理与血清类固醇激素水平或与鱼的大小。在羽田收集的男性壬基酚的平均浓度显着大于男性相模湾。总的来说，这些研究结果表明，内分泌干扰的正常配子发生在东京湾的一些地区的鱼的可能性，这个问题可能是更广泛的比目前recognized.A自动对映体分离系统的开发使用气相色谱制备馏分收集器分离手性化合物的对映体。该系统消除了在通过气相色谱法分离之后对手动收集程序的需要。为了评估该系统的实用性，我们将其应用于分离和收集的对映体的。- 六氯环己烷，我们测量了每种对映体的雌激素效力。的两种对映体。-HCH显示出类似的雌激素活性。我们的结论是，该分析方法适用于测定其他手性化合物的雌激素活性。这种测定是必不可少的，因为对映异构体在生物体中积累和在环境中分解的方式有很大差异。