Study on new source of volatile organoiodine compounds by isolation and culture of marine microorganisms

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The isolation and culture method for marine microorganisms was optimized using a flow cytometer. Bacteria were investigated for the production of halocarbon from natural waters. Bacteria were isolated and incubated at 25ºC, and the concentrations of methyl halides (methyl chloride, methyl bromide, methyl iodide) in the gas phase above cultured samples were determined using dynamic headspace gas chromatography/mass spectrometry. Bacteria isolated from brackish water were identified by 16 Svedberg unit ribosomal-ribonucleic acid (16S rRNA) gene sequence analysis. Five genetically different strains of bacteria belonging to Erythrobacter or Pseudomonas produced methyl chloride, methyl bromide, and methyl iodide for several days in the culture. Maximum production rates for methyl halide were observed in the culture of isolated bacteria (HKF-1) belonging to Erythrobacter. Aquatic bacteria could be a new source of methyl halides in marine environments.