权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on new source of volatile organoiodine compounds by isolation and culture of marine microorganisms

海洋微生物分离培养挥发性有机碘化合物新来源研究

基本信息

批准号：
23310010
负责人：
HASHIMOTO Shinya
金额：
$ 12.4万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

The isolation and culture method for marine microorganisms was optimized using a flow cytometer. Bacteria were investigated for the production of halocarbon from natural waters. Bacteria were isolated and incubated at 25ºC, and the concentrations of methyl halides (methyl chloride, methyl bromide, methyl iodide) in the gas phase above cultured samples were determined using dynamic headspace gas chromatography/mass spectrometry. Bacteria isolated from brackish water were identified by 16 Svedberg unit ribosomal-ribonucleic acid (16S rRNA) gene sequence analysis. Five genetically different strains of bacteria belonging to Erythrobacter or Pseudomonas produced methyl chloride, methyl bromide, and methyl iodide for several days in the culture. Maximum production rates for methyl halide were observed in the culture of isolated bacteria (HKF-1) belonging to Erythrobacter. Aquatic bacteria could be a new source of methyl halides in marine environments.

利用流式细胞仪对海洋微生物的分离培养方法进行了优化。研究了利用天然水体生产卤代烃的细菌。分离细菌并在25ºC下孵育，采用动态顶空气相色谱/质谱法测定培养样品气相中甲基卤化物（氯甲烷、甲基溴、碘化甲酯）的浓度。采用16 Svedberg单元核糖体-核糖核酸（16S rRNA）基因序列分析方法对苦咸水细菌进行了鉴定。属于红杆菌或假单胞菌的五种基因不同的细菌菌株在培养中产生氯甲烷、甲基溴和碘化甲酯数天。在红杆菌分离菌（HKF-1）的培养中观察到最大的甲基卤化物产量。水生细菌可能是海洋环境中甲基卤化物的新来源。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Production of methyl chloride and methyl bromide by Chaetoceros sp.

通过角毛藻生产氯甲烷和溴甲烷。

DOI：
发表时间：
2013
期刊：
影响因子：
0
作者：
Haruka Nishi;Michiko Kurihara;Shinya Hashimoto
通讯作者：
Shinya Hashimoto

Brominated methane compounds and isoprene in surface seawater of Sagami Bay: Concentrations, fluxes, and relationships with phytoplankton assemblages

DOI：
10.1016/j.marchem.2012.04.001
发表时间：
2012-04
期刊：
Marine Chemistry
影响因子：
3
作者：
M. Kurihara;Motoko Iseda;T. Ioriya;N. Horimoto;J. Kanda;T. Ishimaru;Y. Yamaguchi;S. Hashimoto
通讯作者：
M. Kurihara;Motoko Iseda;T. Ioriya;N. Horimoto;J. Kanda;T. Ishimaru;Y. Yamaguchi;S. Hashimoto

Analysis of historical trend of carotenoid concentrations in sediment cores from Lake Shinji

宍道湖沉积物中类胡萝卜素浓度的历史趋势分析