Development of cancer immunotherapy using cancer-specific peptide based on genome analysis

非小细胞肺癌（NSCLC）是导致死亡的主要原因之一。诊断标志物是选择适当治疗所必需的。本研究采用实时荧光定量RT-PCR方法检测了50例非小细胞肺癌（NSCLC）（鳞癌18例，腺癌32例）组织中WT 1基因的表达水平，并分析了WT 1基因表达水平与NSCLC临床病理特征的相关性。统计分析WT 1表达水平与临床状态的相关性，实时荧光定量RT-PCR检测非小细胞肺癌中WT 1的表达水平。在SQ和AD中，WT 1表达与临床状态之间存在显著相关性。SQ组WT 1阳性表达者无淋巴结转移（P=0.038）。

Non-small cell lung cancer (NSCLC) is one of the leading causes of death. Diagnostic markers are required for choices of appropriate treatment. In this study, we examined WT1 expression levels in clinical specimens of non-small cell lung cancers (NSCLCs) and estimated the correlation between the WT1 gene expression levels and clinical statuses.WT1 expression levels of 50 NSCLCs (18 squamous cell carcinoma (SQ), 32 adenocarcinoma (AD)) were examined using quantitative real-time RT-PCR. A correlation between WT1 expression levels and clinical statuses was analyzed by statistical procedures.A wide range of WT1 expression levels was detected in NSCLSs by a quantitative real-time RT-PCR. A significant correlation between the WT1 expression and clinical status was revealed in SQ and AD. SQ patients expressed the WT1 were significantly nonmetastatic to lymph nodes (P=0.038).