Genetic Program Controlling Plant Organogenesis

本项目旨在阐明以拟南芥、水稻、牵牛花、百日草、烟草等高等植物为研究对象的控制植物器官发生的遗传程序。本年度项目取得的主要成果有：(1)突变体的分离：影响花器官发生（冈田，后藤）、分生组织形成（町田，Tasaka）、叶片发育（町田）、根生长（冈田）、根毛发育（冈田）、细胞分裂素独立生长（Kakimoto）、对温度敏感的茎和/或根再生（杉山）和茎向地性（Tasaka）的拟南芥突变体。水稻转座子标签矮化和活孔突变体（Hirochika, Matsuoka）。(2)拟南芥突变体HY5 （Okada）、CAPRICE （Okada）、CKI1 （Kakimoto）、CUP-SHAPED COTYLEDON2 （Tasaka）、SPIRAL1 （Hashimoto）和JAM （Machida）的基因克隆。(3)牵牛花（Iida） MUTABLE基因的遗传分析。(4)叶肉细胞向管状细胞分化的生化和分子分析（Fukuda）。(5)花序特异性基因筛选（Kohchi）。(6)拟南芥（Machida）和水稻（Hirochika）标记系统的产生。研究结果发表在国际科学期刊上，并在会议上报告。

This project aimed to clarify Genetic Program Controling Plant Organogenesis using higher plants such as Arabidopsis, rice, morning glory, Zinnia, and tobacco. Major results obtained in the year project are : (1) Isolation of mutants : Arabidopsis mutants affecting flower organogenesis (Okada, Goto), meristem formation (Machida, Tasaka), leaf development (Machida), root growth (Okada), root hair development (Okada), cytokinin-independent growth (Kakimoto), temperaturesensitive shoot and/or root regeneration (Sugiyama), and shoot gravitropism (Tasaka). Transposon-taggad dwarf, and viviporous mutants of rice (Hirochika, Matsuoka). (2) Gene cloning from Arabidopsis mutants : HY5 (Okada), CAPRICE (Okada), CKI1 (Kakimoto), CUP-SHAPED COTYLEDON2 (Tasaka), SPIRAL1 (Hashimoto), and JAM (Machida). (3) Genetic analysis of MUTABLE gene of morning glory (Iida). (4) Biochemical and Molecular analysis of cell differentiation from leaf mesophyll cells to tracheary elements (Fukuda). (5) Screening of inflorescence-specific genes (Kohchi). (6) Generation of tagging system in Arabidopsis (Machida) or in rice (Hirochika). The results were published in international scientific journals and reported in conferences.