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Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials

Application of Hydrophobic Interaction among Biomolecules under Extreme Conditions to Strongly-correlated Softmaterials
极端条件下生物分子间疏水相互作用在强相关软材料中的应用
批准号:
13031024
负责人:
NAKAMURA Satoshi
金额:
$33.28万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
财政年份:
2001
资助国家:
日本
项目状态:
已结题
起止时间:
2001 至 2003

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中文摘要
翻译
本研究的目的是阐明蛋白质中非共价键对极端环境耐受性的重要性。研究了三角盘状极端嗜盐古菌Haloarcula japonica中的2Fe-2S铁氧还蛋白。H. japonica Fd对耐盐性有重要作用。通过对中等兼性嗜热芽孢杆菌凝结芽孢杆菌(Bacillus coagulans)3-异丙基苹果酸脱氢酶(3-isopropyl malate dehydrogenase,3-IDH)亚基-亚基界面和/或环区进行氨基酸取代,提高了其热稳定性,并对嗜碱芽孢杆菌J813多结构域几丁质酶的几丁质结合结构域的功能进行了初步研究。
英文摘要
The objective of this research is to elucidate the importance of non-covalent bonds in proteins on tolerances towards extreme environments. A 2Fe-2S feredoxin from triangular disc-shaped extremely halophilic archaeon Haloarcula japonica was investigated. It seemed that the additional amino terminal region of H. japonica Fd was importantfor halotolerancy. The thermostability of 3-isopropylmalate dehydrogenase of moderate facultative thermophile Bacillus coagulans was improved by amino acid substitutions at the subunit-subunit interface and/or a loop region.Furthermore, the function of chitin-binding domain of a multi-domain chitinase from alkaliphilic Bacillus sp. J813 was elucidated.
期刊论文(68)
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会议论文
S.Nakamura: "Structure and Function of a Multidomain Alkaline Xylanase from Alkalinhilic Bacillus sp. Strain 41M-1"Catal.Survey Asia. 7. 157-164 (2003)
S.Nakamura:“嗜碱性芽孢杆菌菌株 41M-1 的多域碱性木聚糖酶的结构和功能”Catal.Survey Asia。
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通讯作者:
R.Yatsunami et al.: "A Novel uhitosanase trom bacillus sp. Strain K17 : Gene Cloning and Expression in Escherichia coli"Nucleic Acids Res.Suppl.. 2. 227-228 (2002)
R.Yatsunami 等人:“芽孢杆菌属菌株 K17 中的新型 uhitosanase:基因克隆和在大肠杆菌中的表达”Nucleic Acids Res.Suppl.. 2. 227-228 (2002)
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M.Inami: "Directed Evolution of Xylanase J form Alkaliphilic Bacillus sp. Strain 41M-1"Nucleic Acids Res. Suppl.. 3. 315-316 (2003)
M.Inami:“嗜碱性芽孢杆菌菌株 41M-1 中木聚糖酶 J 的定向进化”核酸研究。
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通讯作者:
八波利恵ほか: "生体内分子の自己修復"科学と教育. 49. 122-125 (2001)
Rie Yamba 等人:“生物分子的自我修复”科学与教育 49. 122-125 (2001)
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共 30 条
    Characterizaition and functional modification of GH family 18 chitinases from alkaliphilic bacteria
    • 批准号:
      16K05839
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.16万
    • 财政年份:
      2016
    • 负责人:
      NAKAMURA Satoshi
    • 依托单位:
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    • 批准号:
      25540080
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
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    • 财政年份:
      2013
    • 负责人:
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    • 依托单位:
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    • 批准号:
      22650018
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.07万
    • 财政年份:
      2010
    • 负责人:
      NAKAMURA Satoshi
    • 依托单位:
    海外基金