Automated screening platform for microscopic plant phenotyping

我们申请资助一个自动筛选系统，用于光合作用有机体动态性状的微观表型鉴定。该平台将由一套四个显微镜和一个机器人系统组成，这些显微镜安装在一个光架上进行培养，机器人系统在培养站和成像站之间运送样本。两个用于大视野自动成像的荧光低放大系统和两个高分辨率共焦显微镜将使基于显微镜的基因筛选成为可能。该平台配备了液体处理系统，可以研究处理的效果或改变生长条件。该系统的关键特征是培养、刺激和成像的完全集成，允许并行地对数百到数千个个体样本的动态反应进行延时记录。

We apply for funding of an automated screening system for microscopic phenotyping of dynamic traits in photosynthetic organisms. The platform will consist of a set of four microscopes that are attached to a light shelf for incubation and a robotic system that transports the samples between cultivation and imaging stations. Two epifluorescence low-magnification systems for the automated imaging of large fields of view and two high-resolution confocal microscopes will enable microscopy-based genetic screens. Equipped with a liquid handling system, the platform will enable studying the effect of treatments or changing growth conditions. The key feature of this system is the full integration of cultivation, stimulation and imaging, which allows for time-lapse recordings of dynamic responses in hundreds to thousands of individual specimens in parallel.