Bio-conversion of Substances and Information by Metal-containing Proteins and Enzymes

我们已经在分子水平上研究了含金属的酶和蛋白质对生物物质和信息的转化，如一氧化氮还原酶、吲哚胺2，3-双加氧酶和氧感受器蛋白。根据快速混合急冷体系捕获的反应中间产物的ESR结果和该酶的晶体结构，提出了NO(一氧化氮)转化为N20(一氧化二氮)的反应机理。根据吲哚胺2，3-双加氧酶的晶体结构，提出了其催化色氨酸代谢的反应机理。我们发现氧感受蛋白可以从CO和NO等其他双原子气体分子中分离出O2，并根据嗜热菌的感受蛋白的结构讨论了分子内和分子间的信号转导途径，这与氧感受蛋白类似。

We have examined conversion of bio-substances and information in molecular level by metal-containing enzymes and proteins such as nitric oxide reductase, indoleamine 2, 3-dioxygenase, and oxygen sensor protein. We proposed the reaction mechansim of conversion of NO (nitric oxide) to N20 (nitrous oxide) on the basis of the ESR results of the reaction intermediates trapped by the rapid mixing-to-quench system, and the crystal structure of this enzyme. We also proposed the reaction mechanism of the tryptophan metabolism catalyzed by indoleamine 2,3-dioxygenase on the basis of its crystal structure. We found that the oxygen sensor protein can descrominate 02 from other two-atomic gas molecules such as CO and NO, and also discussed the pathway of the intra- and inter-molecular signal transduction on the basis of the strcuture of the sensor protein of the thermophilic bacterium, which is analogus to the oxygen sensor protein.