Development of a genome-scale in vitro transcription and translation-coupled system

转录（从DNA合成RNA）和翻译（从RNA合成蛋白质）是所有生物体中普遍存在的系统。利用超嗜热古细菌柯达卡拉热球菌（Thermococcus kodakaraensis）的稳定细胞成分，建立了体外转录系统和体外翻译系统。构建了一个体外转录和翻译耦合反应系统，使体外从DNA模板生产蛋白质成为可能。此外，还实现了由操纵子（作为单个转录单位转录的一组基因）产生蛋白质。

Transcription (RNA synthesis from DNA) and translation (Protein synthesis from RNA) are ubiquitous systems in all living organisms. An in vitro transcription system and an in vitro translation system were developed using stable cell components of a hyperthermophilic archaeon,Thermococcus kodakaraensis. A system for in vitro transcription and translation-coupled reactions was also constructed which enabled in vitro protein production from a DNA template. Furthermore, production of proteins from an operon, a cluster of genes transcribed as a single transcriptional unit, was also achieved.