Studies on molecular assembly of replication proteins and regulation of their functions in initiation of chromosome DNA replication

我们对裂殖酵母染色体上复制起始点进行了全基因组定位，发现着丝粒和交配型基因座的异染色质在S早期复制，而亚端粒的异染色质复制较晚。我们发现，异染色质蛋白Hp1被认为是DNA复制的负面因素，它通过与DNA复制所必需的激酶DDK相互作用，在着丝粒和交配型基因座刺激S早期的复制。另一方面，在亚端粒区域，端粒结合蛋白在DNA复制调控中发挥作用。

We determined whole-genome localization of replication origins on fission yeast chromosomes and found that heterochromatin in centromere and mating type locus replicates in early S phase, whereas that in subtelomere replicates late. We discovered that the heterochromatin protein HP1, which was considered as a negative factor to DNA replication, stimulate replication in early S phase at centromere and mating type locus through the interaction with DDK that is an essential kinase for DNA replication. On the other hand, in subtelomeric regions telomere binding proteins play roles in regulation of DNA replication.