Flow cytometer with up to five lasers, spectral analyses and auto-sampling

我们的研究旨在了解质膜信号通路的空间组织和组装动力学如何控制T细胞活化和功能。这项研究是基于我们的发现，质膜蛋白定位在纳米尺寸的域。我们的研究表明，T细胞活化是由不同纳米结构域的空间关系，其中的信号复合物的组装和它们之间的信号分子的易位动态控制的。我们使用多学科的策略，结合联合收割机单分子显微镜与生物化学，生物物理学和细胞生物学的方法研究潜在的分子机制。具体而言，我们感兴趣的是T细胞受体（TCR）和抑制性程序细胞死亡蛋白（PD-1）在T细胞活化和抗肿瘤反应的途径。我们的最终目标是识别T细胞信号通路中的新控制原理，并利用它们来修改T细胞反应，以用于未来的免疫治疗。我们需要一台带光谱分析仪和自动进样器的流式细胞仪，这对我们在弗赖堡大学新成立的实验室以及实现我们当前和未来的研究目标至关重要。所要求的流式细胞仪对于多种用途至关重要，包括：（i） 研究免疫反应期间和肿瘤环境中的免疫细胞多样性。（二） 分析T细胞发育、分化和活性状态。（三） 对原代细胞和转基因细胞进行质量控制，以最大限度地提高我们研究的成功率和数据，即体内抗肿瘤反应和先进的单分子显微镜。（四） 用于流式细胞术和荧光显微术的新探针和配体的表征和测试。（五） 筛选小分子文库，例如用FRET探针作为读出。流式细胞仪具有以下两个关键能力： 5条激光线（包括紫外线），通过光谱分析检测多达64种荧光团。（二） 96孔板进样器，用于细胞和组织样本以及小分子抑制剂库的中等通量实验。

Our studies seek to understand how the spatial organization and assembly dynamics of plasma membrane signaling pathways control T cell activation and functions. The research is based on our findings that plasma membrane proteins are localized in nanometer-sized domains. Our studies revealed that T cell activation is dynamically controlled by the spatial relationships of distinct nanodomains, the assembly of signaling complexes within them and translocation of signaling molecules between them. We study the underlying molecular mechanisms using multidisciplinary strategies that combine single molecule microscopy with biochemical, biophysical, and cell biological approaches. Specifically, we are interested in T cell receptor (TCR) and the inhibitory program cell death protein (PD-1) pathways during T cell activation and anti-tumor responses. Our ultimate goal is to identify novel control principles in T cell signaling pathways and use them to modify T cell responses for future immunotherapies.We request a flow cytometer with spectral analyzer and autosampler, which is essential to our newly established laboratory at the Albert-Ludwigs-Universität Freiburg and for realizing our current and future research goals. The requested flow cytometer is essential for multiple purposes, including: (i) Studying immune cell diversity during immune responses and in the tumor environment.(ii) Analyses of T cell development, differentiation and activity states. (iii) Quality control of primary and genetically modified cells to maximize success rates and data of our studies, i.e. in vivo anti-tumor responses and advanced single molecule microscopy.(iv) Characterization and testing of new probes and ligands for flow cytometry and fluorescence microscopy.(v) Screening of small molecule libraries, e.g. with FRET probes as read-outs. The flow cytometer has the following two key capabilities:(i) Five laser lines (including UV) for detection of up to 64 fluorophores through spectral analyses.(ii) A 96-wellplate sampler for medium-throughput experiments of cellular and tissue samples, as well as small molecule inhibitor libraries.