Development of novel nucleic acid materials that efficiently work in the intracellular molecular environment

我们利用模拟细胞内分子环境的模型实验系统研究了核酸的相互作用和反应。这项研究为分子拥挤介质对核酸折叠、核酶活性以及与蛋白质和多肽相互作用的影响提供了定量的见解。据推测，这些数据可以弥合在体外和细胞内获得的结果之间的差距。基于有关分子环境影响的结果，我们开发了新的核酸材料和传感方法，可以在不寻常的条件下有效地工作，例如在活细胞中。

We studied nucleic acid interactions and reactions by using a model experimental system that mimics the intracellular molecular environment. This research provided quantitative insights into influences of the molecular crowding medium on nucleic acid folding, a ribozyme activity, and interactions with proteins and peptides. It is supposed that these data can bridge the gap between the results obtained in vitro and in cells. Based on results regarding the effects of molecular environment, we developed novel nucleic acid materials and sensing methods that efficiently work under unusual conditions such as in living cells.