Analysis of intercellular signaling molecules working in organ development and differentiation in plants

为了研究在器官形成和发育中起作用的细胞间信号系统，我们采取了几种方法。我们确定了小的LTP蛋白质质的质外体提取物的花椰菜头有一个作用的形成和维持茎顶端分生组织。遗传学方法揭示，三聚体G蛋白参与调节茎和根分生组织功能的肽激素受体下的细胞内信号转导途径。我们发现一类新的酪氨酸硫酸化糖肽在植物发育过程中作为细胞间信号发挥重要作用。我们研究了microRNA 165/166在形成发育叶片近轴区和远轴区之间的边界中的作用。此外，我们开发了仪器系统，通过使用激光束在单个植物细胞中诱导特定基因或灭活单个细胞

In order to examine intercellular signaling systems working in organ formation and development, we took several approaches. We identified small LTP proteins in apoplastic extracts of cauliflower heads having a role of formation and maintenance of shoot apical meristem. Genetic approach revealed that trimeric G-protein is involved in the intracellular signing pathway under the peptide hormone receptors that regulate shoot and root meristem function. We showed novel group of tyrosine-sulfated glycopeptides had important roles as intercellular signals in plant development. We investigated action of microRNA165/166 in forming the boundary between the adaxial- and the abaxial-regions in developing leaves. In addition, we developed instrumental systems inducing specific gene(s) in a single plant cell or inactivating a single cell by use of laser beams