Functional traits and ecological preferences of soil dwelling vampyrellid amoebae (Vampyrellida, Rhizaria) in a management intensity gradient

原生生物是土壤微生物群的组成部分。它们在食物网中扮演着各种角色，并对环境条件的变化做出快速反应。它们可以通过环境测序来检测，但为了对其影响和作用进行合理的预测，我们需要详细的表型和生态数据。吸血虫变形虫是一种大型的自由生活变形虫，专门捕食其他真核生物。在生物多样性勘探站的土壤中检测到了高丰度和高多样性的它们，但它们的实际生态作用仍然未知。我们将使用现有的数据和DNA样本从生物多样性探索和应用最新的长读测序技术从PacBio测序大部分的核糖体操纵子。由此产生的高质量读数将带来上级系统发育分辨率，并彻底改变吸血鬼的系统发育和分类框架。与此同时，我们将在新鲜的土壤样本中提取和鉴定新的吸血鬼类群，通过光学显微镜和喂养实验。总之，这些数据将导致一个更详细的“生态分类框架”的分配和解释的吸血鬼序列读，并提供了一个基础，完善食物网模型和测试的影响管理强度对吸血鬼社区。

Protists are an integral part of the soil microbiota. They play various roles in food webs and react quickly to changes in environmental conditions. They can be detected by environmental sequencing, but for sound predictions of their impact and roles, we need detailed phenotypic and ecological data. The vampyrellid amoebae are a large order of free-living amoebae, which specifically prey on other eukaryotes. They have been detected in soil from the Biodiversity Exploratories in high abundances and diversity, but their actual ecological roles remain unknown. We will use the existent data and DNA samples from the Biodiversity Exploratories and apply the newest long-read sequencing techniques from PacBio to sequence large parts of the ribosomal operon. The resulting high-quality reads will bring superior phylogenetic resolution and revolutionise the vampyrellid phylogeny and taxonomic framework. In parallel, we will work on fresh soil samples to extract and characterise new vampyrellid taxa by light microscopy and feeding experiments. Together, these data will result in a much more detailed “eco-taxonomic framework” for the assignment and interpretation of vampyrellid sequence reads, and provide a basis for refining food web models and testing the effect of management intensity on vampyrellid communities.