Immobilised Lipid Chromatography for Membrane Protein Isolation and Analysis

目前的膜蛋白技术不足以应对新出现的蛋白质组挑战，预计大约40%的蛋白质是与膜相关的。这项建议的目的是开发一种新的方法，利用我们最近开发的固定化膜层析材料来纯化膜蛋白。本提案将提供一种新的高分辨率分离技术，该技术可以分离和在线分析复杂的膜蛋白混合物，用于随后的蛋白质组分析、高通量筛选或结构研究，并可能为进一步开发新的商业膜蛋白分析工具奠定基础。

Current techniques for membrane protein are inadequate for the emerging proteomic challenge, in which approximately 40% of proteins are predicted to be membrane associated. The aim of this proposal is to develop a new approach to purify membrane proteins using our recently-developed immobilised membrane chromatography materials. The present proposal will provide a new high-resolution separation technique that allows isolation and on-line mass analysis of complex mixtures of membrane proteins for subsequent proteomic analysis, high-throughput screening or structural studies and could form the basis for further development of new commercial tools for membrane protein analysis.