5-Laser Flow cytometer

在生物医学研究领域，多参数流式细胞术是阐明免疫生物学基本过程的不可缺少的技术，是开发新的治疗方法的基础。免疫学方面正在最不同的研究方向中脱颖而出，例如神经学、衰老和创伤研究领域，以及肿瘤学基础和翻译研究--研究地点乌尔姆的焦点。能够开展前沿研究，不仅需要优秀的思想和理念，还需要高度专业化的装备。这里使用的高性能流式细胞仪(5个激光器，30个荧光探测器，2个散射光探测器)需要全面地从表型和功能上表征稀有和复杂的细胞群。此外，样品材料的局限性要求同时分析大量的参数。在单细胞和种群水平上对转录组进行平行研究也产生了对异种免疫细胞种群进行全面表征的需要。参与这一应用的工作组的项目绝对需要存在这里应用的高性能流式细胞仪，以便能够实现面向基础和平移的研究项目，并能够进一步发展到国际上和具有竞争力。对高性能流式细胞仪的高需求和高昂的采购成本表明，支持将该设备整合到现有的中央核心设施“免疫监测”中，为所有感兴趣的科学家提供在所有机构的人和鼠免疫监测领域的最佳全面服务。该设备集成在中央核心设施“免疫监测”中，能够有效地使用该设备，并为用户提供从规划到实施和评估所生成数据的专家支持。目前，配备Beckman Coulter(Gallios Flow Cytometer；10 Colors)的3激光装置的核心设施“免疫监测”，已经达到了处理电流和创造性问题的极限。使用更强大的分析型流式细胞仪，配备了5个激光，可以检测多达30种颜色，大大提高了研究结果的质量。此外，这为实施复杂的、当代的和创新的研究方法提供了机会。这使我们能够取得具有治疗意义的重大科学成果，另一方面，我们能够坚持竞争，争取获得国家和国际联合和个人筹资措施。

In the field of biomedical research, multi-parameter flow cytometry represents an indispensable technology for the clarification of fundamental immunobiological processes as a basis to develop new therapeutic approaches. Immunological aspects are coming to the fore in the most diverse directions of research, such as in the fields of neurology, aging and trauma research as well as in oncological basic and translational research - focal points of the research location Ulm. Being able to carry out cutting-edge research requires not only outstanding ideas and concepts, but also highly specialized equipment. The high-performance flow cytometer applied here (5 lasers, 30 fluorescence detectors, 2 scattered light detectors) is required to comprehensively - phenotypically and functionally - characterize rare and complex cell populations. Furthermore, the limitation in the sample material requires a simultaneous analysis of a large number of parameters. The need for a comprehensive characterization of heterogeneous immune cell populations also arises from the parallel investigation of the transcriptome at the single cell and population level. The projects of the working groups involved in this application absolutely require the existence of a high-performance flow cytometer as applied here in order to be able to realize the basic-oriented and translational research projects and to be able to further develop internationally and competitively.The high demand for a high-performance flow cytometer and the high acquisition costs speak in favor of integrating this device into the existing central core facility "Immune Monitoring", which offers all interested scientists optimal full service in the field of immune monitoring of humans and mice across all institutions. The integration of this device in the central core facility "Immune Monitoring" enables efficient use of the device as well as expert support for the user from planning to implementation and evaluation of the generated data. At present, the core facility "Immune Monitoring", equipped with a 3-laser device from Beckman Coulter (Gallios Flow Cytometer; 10 colors), has reached the limits of processing current and creative questions. The use of a more powerful analytical flow cytometer, equipped with 5 lasers and the possibility of detecting up to 30 colors, significantly increases the quality of the research results. Moreover, this opens up opportunities to implement complex, contemporary and innovative research approaches. This enables us to achieve therapeutically significant scientific results and on the other hand, to be able to persist in the competition for the acquisition of national and international joint and individual funding measures.