High-resolution tandem mass spectrometer

介绍了一种用于复杂生物和合成样品的非靶向分析的超高效液相色谱联用高分辨率串联质谱仪。因此，要求具有高分辨率、高质量精度、高质量稳定性、高扫描速度和宽动态范围的质谱仪。为了能够识别未知和已知的化合物，仪器应该能够获得高分辨率的质量，以及MS/MS光谱。因此，采用电喷雾(ESI)离子源和四极飞行时间探测器(Q-ToF)的串联质谱计。该液相色谱系统应包括一个二元高压泵、一个带冷却系统的自动进样器和一个二极管阵列检测器。除了无针对性的分析问题外，该仪器还将用于新型酶的生化表征，以及蛋白质工程项目中酶变异体的活性筛选。

A high-resolution tandem mass spectrometer with ultra high performance liquid chromatography coupling for the untargeted analysis of complex biological and synthetic samples is applied for. Therefore, a mass spectrometer with high resolution, mass accuracy, mass stability, scan rate and a broad dynamic range is requested. To enable identification of unknown and known compounds, the instrument should enable acquisition of the high resolution mass, as well as MS/MS-spectra. Thus, a tandem mass spectrometer with electronspray (ESI)-ionsource and a quadrupol- time-of-flight detector (Q-ToF) is applied for. The liquid chromatography system should include a binary high pressure pump, an autosampler with cooling system, and a diode array detector. Besides untargeted analytical questions, the instrument will be utilized for the biochemical characterization of novel enzymes, and the activity-screening of enzyme variants for protein engineering projects.