Functional Characterisation of Myosin Motors (Funktionelle Charakterisierung von Myosinmotoren)

重组肌球蛋白的产生和表征将是我们项目对该计划的主要贡献之一。我们将专门设计和优化肌球蛋白和肌球蛋白片段用于动力学，结构和细胞生物学研究。这种方法将使我们能够将特定突变引入的结构改变与肌球蛋白马达的体内和体外功能联系起来。主要的问题，可以解决与这种方法是：（1）如何突变肌动蛋白结合的肌球蛋白电机面影响与肌动蛋白的相互作用？(2)肌动蛋白结合面的突变如何改变运动其他领域的事件？(3)核苷酸结合位点、肌动蛋白结合面和杠杆臂之间的信息是如何传递的？(4)通过用合成序列交换表面环或结构模块，马达的酶性质能否以可预测的方式改变？(5)非常规肌球蛋白的酶学性质与肌球蛋白II有何不同？

The generation and characterisation of recombinant myosins will be one of the major contributions of our project to the programme. We will specifically design and optimise myosins and myosin fragments for use in kinetic, structural and cell biological studies. This approach will allow us to link structural alterations introduced with specific mutations to the in vivo and in vitro functions of the myosin motor. Major questions that can be addressed with this approach are: (1) How do mutations in the actin-binding face of the myosin motor affect the interaction with actin? (2) How do mutations in the actin-binding face alter events in other domains of the motor? (3) How is information communicated between the nucleotide-binding site, the actin binding-face, and the lever arm? (4) Can the enzymatic properties of the motor be altered in a predictable way by exchanging surface loops or structural modules with synthetic sequences? (5) How do the enzymatic properties of unconventional myosins differ from those of a myosin II?