Imaging of properties of coherent elastic light scattering from turbid biological media

用相干的、弹性散射光成像来观察体内的厚组织形态，或者深埋在浑浊介质中的细胞，仍然是一个主要的挑战。我们采用另一种方法的成像性质的光散射基于将细胞组织作为一个空间变化的折射率连续体编码散射光。我们提出了基于相位延迟、散斑和角分辨散射间接推断组织状态和形态的新方法。我们将在原位上皮组织和细胞的结构和功能与光散射之间的关联方面取得新的突破，例如，在厚浊介质中埋藏的细胞中检测有丝分裂和凋亡，以及体内的癌症和癌前病变。

Imaging with coherent, elastically-scattered light for visualisation of thick-tissue morphology in vivo, or of cells buried deep in a turbid medium, remains a major challenge. We adopt an alternative approach of imaging of properties of light scattering based on regarding cellular tissue as a spatially-varying refractive-index continuum which encodes scattered light. We propose new methodologies to infer tissue state and morphology indirectly based on phase delay, speckle, and angle-resolved scattering. We will break new ground in correlating the structure and function of in situ epithelial tissue and cells to light scattering enabling, e.g., the detection of mitosis and apoptosis in cells buried in thick, turbid media, and of cancers and precancers in vivo.