4-Laser Cell Sorter

基尔大学生物系专注于实验进化、微生物组的操纵和宿主细胞反应的评估、细菌的分离和分化以及单细胞转录。不同工作组的更大的资金倡议和几个单独的项目旨在揭示宿主-微生物的相互作用和进化。在基尔大学的生物学中心，我们目前缺乏一种超越了通过流式细胞术对细菌和真核细胞进行量化的仪器，并且可以同时对原核细胞和真核细胞进行分选。购买荧光激活的细胞分选器将大大提高我们在基尔大学的实验能力，并将我们在操纵有机体相互作用和细胞反应方面带到一个新的水平。使用流式细胞仪将有助于a)了解和跟踪单个或多个细菌菌株以及它们在加入荧光标记的细菌菌株和病毒后在微生物群中的相互作用；b)在进化实验中对竞争菌株进行量化，并在单个菌株分选后重新分离它们，这将使进一步培养或例如对宿主的感染实验能够在实验进化后评估毒力的变化；c)对宿主细胞进行量化和分类以启动细胞培养或利用单细胞转录组分来评估它们的发育或基因表达；以及d)评估细胞信号和成分的表达，以及e)分析细胞复制和细胞周期。

The department of Biology at Kiel University has a strong focus on experimental evolution, manipulation of the microbiome and the assessment of host cellular responses, the isolation and differentiation of bacteria, and single cell transcriptomics. Larger funding initiatives and several individual projects of a diversity of working groups aim to unravel host-microbe interactions and evolution. In the Biology Center of Kiel University, we currently lack an instrument that goes beyond the quantification of bacteria and eukaryotic cells over flow cytometry, and that would permit sorting of both prokaryotic as well as eukaryotic cells. The purchase of a Fluorescence Activated Cell Sorter would significantly advance our experimental capabilities at Kiel University and bring us on a new level in terms of manipulation of organismic interactions and cellular responses. The usage of the FACS would facilitate to a) understand and follow single or multiple bacterial strains and their interaction in the microbiome upon adding fluorescently labelled bacteria strains and viruses, b) the quantification of competing bacterial strains in evolution experiments and their re-isolation upon sorting of the single strains, that would permit further cultivation or e.g. infection experiments on the host to assess shifting virulence upon experimental evolution, c) quantify and sort host cells to start cell cultures or to assess their development or gene expression using e.g. single cell transcriptomics, and d) assess the expression of cellular signals and components and e) analyse cellular replication and cell cycle.