Histochemical study of the fine distribution and development of microcirculatory system.

对微循环系统、淋巴管和血管进行组织化学研究，探讨淋巴管毛细血管网结构组织的器官特异性和精细分布。淋巴和血管的特征是用酶组织化学方法，除免疫组织化学方法外，还使用5'-核苷酸酶（5'-Nase）、碱性磷酸酶（ALPase）和/或二氨基肽酶（DAPase）染色。5'- nase阳性淋巴管可根据ALPase和DAPase活性分别与动脉和静脉血管进行组织化学区分。在光镜和扫描电镜或透射电镜下对同一标本进行比较组织化学研究，证实酶反应的特异性和定位。此外，本报告提出了一种新的适用于扫描电镜组织化学重金属染色的淋巴管5'-Nase反应和神经丛乙酰胆碱酯酶反应的背散射电子成像。这些组织化学方法讨论了它们在正常和病理条件下证明血管网络器官特异性的能力。

The microcirculatory system, lymphatics and blood vessels was histochemically studied in regards to the organ specificity of the structural organization and fine distribution of the lymphatic capillary network. The lymphatics and blood vessels are characterized by an enzyme-histochemical method using 5'-nucleotidase (5'-Nase), alkaline phosphatase (ALPase) and/or diaminopeptidase (DAPase) staining in addition to an immunohistochemical method. The 5'-Nase-positive lymphatic vessels can be distinguished histochemically from arterial and venous vessels based on ALPase and DAPase activity, respectively. The specificity and localization of enzyme reactions were confirmed by comparative histochemical studies of the same specimen under light microscope and scanning or transmission electron microscope. Furthermore, this report presents a new applicability of the backscattered electron imaginings of 5'-Nase reaction for lymphatics and acetyicholinesterase reactions for nerve plexus to scanning electron microscopic histochemistry stained with heavy metal, respectively. These histochemical methods are discussed in relation to their ability to demonstrate the organ specificity of vascular networks under normal and pathological condition.