High resolution confocal microscope

代表生物和生物技术学院，行为神经科学工作组想申请一台高分辨率共聚焦显微镜来分析神经退行性疾病的潜在细胞机制和神经回路。行为神经科学工作组和普通动物与神经生物学部门长期以来一直对鉴定不同的G蛋白偶联第二信使和离子通道感兴趣，这些通道负责在活组织和行为小鼠中导致衰弱性疾病，如共济失调、癫痫、创伤后应激障碍和失明。我们创建并利用现有的小鼠模型，以人类疾病，发作性共济失调2型，脊髓小脑性共济失调6型和视网膜变性来了解这些疾病状态的潜在机制。通过结合应用最先进的光遗传学、化学遗传学、基因编码荧光传感器和钙成像、电生理学和行为技术，我们有强大的装备来解决和解剖这些复杂的疾病状态，这将从高分辨率显微系统中受益匪浅。高分辨率设备将实现亚细胞细胞器和聚集体的长期活细胞成像（高分辨率），表达多达6种不同的荧光蛋白（光谱分离/解混），同时去除自身荧光（自身荧光校正功能）。目前，生物与生物技术学院对高分辨率共聚焦显微镜的需求很大，而现有的共聚焦显微镜无法满足这一需求。所要求的高分辨率显微镜将是一个多用户设施，并可供生物和生物技术和信息学部门以及波鸿鲁尔大学的所有教职员工使用。

On behalf of the Faculty of Biology and Biotechnology, the Working Group of Behavior Neuroscience would like to apply for a high-resolution confocal microscope to analyze the underlying cellular mechanisms and neural circuitry underlying neurodegenerative diseases. The Working Group of Behavior Neuroscience and Department of General Zoology and Neurobiology have a long-standing interest in identifying the different G protein coupled second messenger players and ion channels responsible for debilitating diseases such as ataxia, epilepsy, post-traumatic stress disorder and blindness in living tissues and behaving mice. We created and utilize available mouse models to the human diseases, episodic ataxia type 2, spinocerebellar ataxia type 6 and retinal degeneration to understand the underlying mechanisms of these diseased states. By applying a combination of state-of-the-art optogenetic, chemogenetic, genetically encoded fluorescent sensor and calcium imaging, electrophysiology and behavioral techniques, we are strongly equipped to tackle and dissect out these complex disease states which would highly benefit from a high-resolution microscopic system. A high-resolution device would enable long term live cell imaging from subcellular organelles and aggregates (high-resolution) expressing up to 6 different fluorescent proteins (spectral separation/unmixing) and simultaneously removing autofluorescence (autofluorescence correction feature). Currently there is a high demand and need for a high-resolution confocal microscope in the Faculty of Biology and Biotechnology which our current confocal microscope cannot fulfill. The high-resolution microscope requested will be a multi-user facility and be available for all faculty members in the Departments of Biology and Biotechnology and Informatics, and at the Ruhr-University Bochum.