Role of histamine receptor ( <H_1> , <H_2> ) positive cells in contact hypersensitivity reaction (CS).

用4-羟基-3-硝基苯乙酰(NP)诱导大鼠足底肿胀，研究了组胺(H)在CS中的免疫调节机制。在诱导期对CS有明显的抑制作用，而在效应期则无明显抑制作用。这种抑制作用不受H-2基因的限制，可被H_1&GT和H_2受体(R)拮抗剂消除。H注射小鼠的非尼龙柱贴壁细胞在NP免疫的小鼠体内具有抑制CS的能力。这些细胞膜表面有Thy1.2、Ly1、Ly2和I-J抗原。用FITC标记的H(FHH)标记的人血清白蛋白对P388&lt；D_1&Gt；细胞进行库尔特EPICS-C细胞分选。FHH与P388、D_1、GT；细胞膜结合，但对细胞表面结合无竞争性抑制作用，胞浆内cAMP水平在正常范围内。

Immuno modurating mechanism of histamine (H) in CS was studied by footpad swelling induced with 4-hydroxy-3-nitrophenyl acetyl (NP). CS was markedly suppressed by H i.p.inj in induction phase but not in effector phase. This suppression was not ristricted by H-2 genes, and was eliminated by <H_1> and <H_2> receptor (R) antagonist. Non nylon column adherent cells from H injected mouse had capacity to suppress CS in NP immunized mice to which these cells were transfered. These cells had Thy1.2, Ly1, Ly2, and I-J antigens on membrance surface. Study on HR of P388 <D_1> cells was done by Coulter EPICS-C cell sorting using FITC labeled human serum albumin conjugated with H (FHH). Though FHH binded to P388 <D_1> cell membrane, H did not showed competitve inhibition of FHH binding to cell surface, and cyclic AMP level in these cells cytoplasma was in normal range.