Role of immunoglobulin receptors on basophil and neutrophil in bronchial asthma.

为探讨迟发性哮喘的发病机制，本文应用免疫扫描电镜、透射电镜、组胺和白三烯分析等方法，研究了循环和气道嗜碱性粒细胞和中性粒细胞免疫球蛋白受体的作用。此外，还使用相同的技术研究了免疫治疗期间IgG抗体数量增加的影响。(1)用免疫电镜技术证实人外周血嗜碱性粒细胞上存在IgE和IgG受体。IgG受体在某些部分是IgG 4受体，其数量少于IgE受体。这些IgG受体不呈斑块状或帽状分布，与嗜碱性粒细胞的活化无关。本研究中，未能证实其上存在IgG 1受体。(2)IgE介导的组胺释放百分比和反应性嗜碱性粒细胞的发生率高于IgG介导的反应。然而，一些难治性哮喘患者的嗜碱性粒细胞在哮喘发作后仍有活性。 ...更多信息 通过IgG或IgG 4介导的反应比通过IgE介导的反应更易发生。(3)嗜碱性粒细胞肥大细胞和中性粒细胞高度观察痰和支气管肺泡灌洗液从顽固性哮喘。与非难治性哮喘患者相比，钙离子载体抗IgG抗体刺激可加速外周血中性粒细胞产生白三烯，但抗IgE抗体似乎不产生白三烯。念珠菌提取物也能诱导产生IgG抗体，在许多难治性哮喘患者中检测到IgG抗体，高于屋尘提取物。(4)免疫治疗后嗜碱性粒细胞反应性和IgE/IgG受体比值下降。IgG 4骨髓瘤蛋白被动致敏后的嗜碱性粒细胞对抗IgE的反应减弱，抗原（抗IgE抗体）与受体上IgE抗体的结合减弱。这些数据表明，IgG 4抗体对IgE介导的嗜碱性粒细胞表面反应具有抑制作用。少

To clarify the pathogenesis of late-onset intractable asthma, role of immuno-globulin receptors on basophils and neutrophils in circulation and airway was studied by immuno-scanning and -transmission electron microscopy, histamine and leukotrienes analysis. Moreover, effect of IgG antibody increased in number during immunotherapy was also investigated using same techniques.(1) Residence of IgE and IgG receptors on human peripheral blood basophils were proved using a technique of immuno-electron microscopy. IgG receptors which were IgG4 receptors in some part were less in number than IgE receptors. These IgG receptors didn't show a redistribution of patch or cap formation, and had no relation with activation of basophils. In this study, we couldn't get a prove of IgG1 receptor on it.(2) Percent histamine release and incidence of reactive basophils were observed by IgE-mediated reaction higher than by IgG-mediated reaction. However, basophils from some of intractable asthmatics were acti … More vated by IgG- or IgG4-mediated reaction more than by IgE-mediated reaction.(3) Basophil-mast cells and neutrophils were highly observed in sputum and bronchoalveolar lavage fluid obtained from intractable asthmatics. Production of leukotrienes from peripheral blood neutrophils by stimuli of Ca ionophore anti-IgG antibody were accelerated in such cases compaired with non-intractable asthmatics, but no production was seem by anti-IgE antibody. The production was also induced by candida extract, which antibodies of IgG fraction were detected in many intractable asthmatics, higher than by house dust extract.(4) Reactivity of basophils and ratio of IgE/IgG receptors on the cell were decreased after immunotherapy. Basophils after passive sensitization with IgG4 myeloma protein got less response to anti-IgE and became less binding of antignens (anti-IgE antibody) to IgE antibody on the receptor. These data indicate that IgG4 antibodies act a role of inhibition of IgE-mediated reactions on the surface of basophils. Less