Experimental study on the regeneration of nerve

用电子显微镜和荧光显微镜观察了大鼠坐骨神经挤压损伤和神经移植后膈膜的再生和通透性。1)损伤后1周和2周，成纤维细胞样细胞呈同心排列，约10层。损伤后4周和8周，扁平的细胞覆盖在由一层或两层组成的神经束上，这些细胞具有基底膜和许多胞饮泡。损伤后24周和36周，会神经膜再生为正常结构。在神经束中，损伤一周后，再生神经纤维周围的成纤维细胞样细胞开始形成隔室。损伤后4周，这些细胞有基底膜和许多胞饮囊泡。8周后，这些细胞的突间连接紧密，具有神经周围细胞的形态特征。2)在神经膜再生过程中，神经内膜成纤维细胞获得了神经膜的形态特征，如微胞囊泡、基底膜连续、细胞附着等。在移植物和远端神经干中，神经内膜成纤维细胞在神经周围间隙增殖并延长其突起，并随着再生过程的发展分化为神经周围细胞。在近端和远端缝合线处，神经内膜成纤维细胞形成小束，这些成纤维细胞环绕着再生轴突和雪旺细胞束，并分化为神经周围细胞。

A study was made on the regeneration and the permeability of the prineurium following crush injury and nerve grafting to rat sciatic nerves by electron and fluorescence microscopes.1) One and two weeks after injury, fibroblast-like cells showed a concentric arrangement consisting of approximately 10 layers. Four and eight weeks after injury, the flattened cells which had basement menbranes and many pinocytotic vesicles, covered the fascicle consisting of one or two layers. Perineurium was regenerated as normal structure 24 and 36 weeks after injury. In the nerve fascicle, one week after injury, compartment began to be formed by fibroblast-like cells around the regenerated nerve fibers. Four weeks after injury, these cells had basement menbranes and many pinocytotic vesicles. Eight week, these cells showed tight junctions between their processes, and had morphological characteristics of perineurial cells.2) In the regeneration of perineurium, endoneurial fibroblasts were observed to gain morphological characreristics of perineurium, such as micopinocytic vesicles, continuous basement membrane and cell attachment. In the graft and distal nerve trunk, endoneurial fibroblasts proliferated and elongated their processes in the subperineurial sapaces, and differentiated into perineurial cells as the regenerating processes developed. In the proximal and distal suture lines, minifascicles were formed by endoneurial fibroblasts, which encircled bundles of regenrating axons and Schwann cells and differenciated into perineurial cells.