Development of the enzyme-linked immunosorbent assay kit for sperm-immobilizing antibodies.

我们通过使用临床定义的精子固定抗体不育妇女的血清样本，鉴定了一种独特的精子细胞膜抗原，其分子大小为15,000道尔顿。抗原对酸、碱和各种蛋白水解酶的稳定性检测表明，该抗原表位可能是糖蛋白的多肽部分。抗该分子的抗血清可抑制精子活力和精子与卵子的相互作用。为建立快速、可靠的抗精子抗体检测体系，对市售抗精子抗体酶联免疫吸附试验(ELISA)试剂盒与标准精子固定试验进行了比较。然而，这些方法检测到不同的抗体活性谱，尽管往往是重叠的。

We have identified a unique sperm cell membrane antigen with a molecular size of 15,000 daltons by using clinically defined serum samples from infertile women with sperm immobilizing antibodies. The stability examination of the antigen against acid, alkali and various proteolytic enzymes indicated that the antigen epitope could be a peptide portion of the glycoprotein. The antisera against this molecule could inhibit sperm motility as well as sperm-egg interaction. In order to develop a rapid reliable assay system for antisperm antibodies, a commercially available enzyme-linked immunosorbent assay (ELISA) kit for antisperm antibodies was compared with standard sperm immobilization test. However, these methods detect a different, though often overlapping spectrum of antibody activity.