Environmental Control in Biological Engineering

本研究开发了超声喷雾法对胚胎细胞和组织培养血管的自动筛选系统。通过愈伤组织阶段发育胡萝卜体细胞胚，并通过两种筛法筛选。采用不同的方式切换液体介质的流动，以防止网格堵塞。一个实验由四个连续的试验组成。虽然在前两次实验中不能获得很多细胞，在第三次试验中鱼雷阶段细胞与超过300微米的总细胞的比率是163/683。结果表明，正常细胞比例较高，网格阻塞不严重。采用大花蕙兰和马铃薯进行喷雾栽培。结果表明，水培大花蕙兰鲜重与水培大花蕙兰鲜重差异不大，虽然水培大花蕙兰干重较差，但水培大花蕙兰对糖和水分的吸收可以通过水培系统控制。在马铃薯栽培中，所有蔬菜在喷雾系统中生长均优于其他系统。虽然通过喷雾间隔可以很容易地控制水和糖的吸收，但必须解决容器内的高湿度条件以及容器表面水滴引起的低光强。

Automatic screening system for enbryogenic cells and tissue culture vessels by means of ultrasonic misting have been developed in the present research year.Through the stage of callus, carrot somatic embryos were developed and they were screened through two kinds of meshes. the flow of liquid media was switched in different ways in order to prevent blocking of meshes.One experiment consisted of four continuous trials. Although not many cells can be obtained in the first two experiments, in the third trial the ratio of cells in the torpedo stage to the total cells over 300 micron meter was 163/683. It is concluded that the ratio of normal cells is relatively high and the blocking of meshes is not serious.Cymbidium PLB and potatoes were used for misting culture. It was found that there is no difference between the fresh weight of Cymbidium PLB in misting culture and that of liquid culture and absorption of sugar and water can be controlled by misting system although the dry weight in misting system is inferior.In potato cultivation, all vegetable growth in misting system is superior to the other.It would be necessary to solve high humidity condition in vessels as well as lower light intensity caused by water droplets on the vessel surfaces although the absorption of water and sugar is easily controlled by misting intervals.