Identification of transforming gene and its products of Marek's disease virus.

1)用马立克病病毒血清型1 （MDVl）感染鸡胚淋巴细胞，在白细胞介素2的作用下进行培养和后续培养，获得持续增殖的t淋巴母细胞样细胞MDCC-MTBl。该细胞系在雏鸡体内具有高度可移植性，并可在不同器官内形成实性淋巴瘤。移植的MTBl细胞在鸡脾细胞培养上获得了增殖细胞。用mdvl特异性磷酸化多肽（可能是T抗原）单克隆抗体免疫荧光检测MTBl细胞P抗原的表达，发现IUdR处理后的细胞移植到鸡体内后P抗原阳性细胞的比例显著增加。我们鉴定了致癌的mdl特异性DNA结构（BamHI-D和-H）。在MTBl细胞中也发现了这些结构。Northern blot杂交结果显示，主要的poly(A) mRNA为2.3 kb，较少的poly(A) mRNA为4.6 kb。

1). Continuously proliferating T-lymphoblastoid cells, named MDCC-MTBl, were obtained by infection of chick embryo lymphocytes with marek's disease virus serotype 1 (MDVl), in cluture and subsequent cultivation in the presence of interleukin 2. This cell line was highly transplantable in chicks, and formed solid lymphomas in various organs. Proliferating cells derived from the transplanted MTBl cells were obtained on culture of the spleen cells of this chick. When the MTBl cells were examined for P antigen expression by immunofluorescence test with the monoclonal antibody against MDVl-specific phosphorylated polypeptides, which seems to be T antigen, it was found that the percentage of P antigen-positive cells after the treatment with IUdR was significantly increased by the transplantation of the cells into chicks.2). We identified oncogenic MDVl-specific DNA structures (BamHI-D and -H). These structures were also identified in MTBl cells. Northern blot hybridization revealed that the major poly(A) mRNA of 2.3 kb and the less abundant one of 4.6 kb were encoded from these regions.